In present work, new tetra-dentate ligand, titled 3,5-bis ((E)-5-Bromo-2-hydroxy benzylidene amino) benzoic acid (H 3 L), was prepared via an acid-catalyzed condensation process. New four metallic ligand complexes with Co(II), Ni(II), Cu(II) and Zn(II) ions, were also prepared from the refluxing of equivalent moles. Ligand's structure and its complexes; were confirmed by numerous characterization methods, including Ultraviolet-Visible, Infrared, Mass Spectrometer, 1 H and 13 C Nuclear Magnetic Resonance spectra, atomic absorption, magnetic moments, and molar conductivity measurements. The results of the spectroscopic analyzes proved that the prepared ligand acts as tetradentate bi-ionic ligand and it was bonded to the metal ions by two nitrogen atoms of the two azomethine groups and by two oxygen atoms of the two phenolic hydroxyl groups after losing their two protons. Octahedral structure proposed to all prepared complexes. The (anti-bacterial) and (anti-fungal) activities of these compound were screened against (E. coli, S. aureus, Klebsiella spp., S. epidermidis,), and (Candida albicans). The results indicated that these compounds have moderated inhibition behavior. The activity of the prepared compounds against Acetyl Choline Esterase Enzyme (AChE) have also studied and the obtained data indicated the presence of different inhibition behavior.

A simple, sensitive and precise colorimetric method for uric acid determination was described. The method based on the complexion reaction between uric acid, potassium ferricyanide, and ferric chloride in hydrochloric acid medium to form blue colored charge transfer complex measured at maximum wavelength 752 nm. The optimum conditions obtained were volume 0.3 ml for hydrochloric acid, 1.25 ml for both potassium ferricyanide and ferric chloride solutions, 60•C and 8 minutes as maximum temperature and time of reaction, the order of mixing additives was uric acid, ferric chloride, potassium ferricyanide, and hydrochloric acid. The highly accurate and precise results obtained with RSD%, Recovery%, Ere%, and D.L were (0.755 to 4.376), (99.676 to 97.831), (-0.033 to -2.169), and 0.1569 µg mL -1 respectively, the calibration graphs were linear in the concentration range of (2.0-200µg ml -1 ), the . molar absorptivity (5937.473L mol -1 cm -1 ) The proposed methods were successfully applied for the determination of uric acid in serum and urine samples for twenty persons.

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