The diagnosis of toxoplasmic infection in aborted women and congenitally infected infants suspected to have toxoplasmosis infection can be difficult due to similarity symptoms with other diseases. A combination of symptoms, serology and polymerase chain reaction (PCR)may facilitate diagnosis of toxoplasmosis in some patients. The present study compare the detection of toxoplasmosis infection by ELISA IgA and IgG antibodies with Real Time polymerase chain reaction (RT-PCR)in the study subjects. A total of 81 sera samples, 57(70.3%) samples from aborted women and 24(29.7%)samples from congenitally infants have been studied. 49(86%) samples from the aborted women were positive and 8(14%) samples were negative as diagnosed by one or two of ELISA markers (IgAand IgG).The ELISA results indicated that 15(62.5%) samples from infants were positive and 9(37.5%) samples of them were negative. RT-PCR tests indicated that 33(67.3%) from the mothers and 6(40%) from the infants were agreed with ELISA positive samples. For ELISA negative samples, RT-PCR detected toxoplasmosis DNA in 4 (50%) and 2 (22.2%) for the mothers and infants respectively. Therefore, ELISA and RT-PCR can make a good combination tests in detection toxoplasmopsis infection.

In order to achieve precise diagnosis of toxoplasmosis in suspected cases of aborted women and infants with abnormal clinical manifestation, 1725 serum samples consisting of 1450aborted women and 77 infants were tested by enzyme linked immune sorbent assay (ELISA), using IgG, IgM and IgA platellat oxogondii kits, 865 (56.64%) were found positive, 848 ( 58.48% ) were found in aborted women and 15 (22.07%) infants with a significant differences (P≤ 0.05).The immunoglobulin IgM , were detected in 72 (8.49%)cases of aborted women, IgM with IgG were found in 44 (61.11%) and with IgA in two (2.77%) cases ,whereas found in three(17.64%)cases of infants. While IgA were found in 77(9.08%) cases of aborted women, and 6 (35.22%) infants. IgG was detected in966 (82.42%(with a significant differences(p≤ 0.05 ),alone in602 (86.12%) ,and with IgA in 34(4.86%) case of aborted women and 8(47.05% )of infants. These results were support the importance of testing the suspected cases by all kinds of immunoglobulins (IgG, IgM and IgA) to achieve precise diagnosis for toxoplasmosis.

No abstract

Toxoplasma gondii has a worldwide distribution and it is one of the most prevalent infectious agents in Iraq. The study was conducted on 200 serum samples of unmarried female university of students age ranged between 18 to 26 years to detect Toxoplasma gondii antibodies. The aim of this study was to detect T. gondii antibodies among unmarried female students in Iraqi universities using different serological tests. Seventy six (38%) serum samples out of 200 subjects were positive for toxoplasma antibodies by Latex agglutination test (LAT). Among 76 LAT sera positive ,only 58 (29%) serum samples were positive with toxoplasma IgG ELISA test , however , the results of IgM ELISA assay were positive only for 3 (1.5%) unmarried female sample .None of negative LAT serum samples gave positive results with neither IgG nor IgM ELISA.