A comprehensive study on the orchid diversity in Penang Hill, Penang, Malaysia was conducted from 2004 to 2008 with the objective to evaluate the presence of orchid species listed by Curtis (J Strait Br R Asiat Soc 25:67-173, 1894) after more than 100 years. A total of 85 species were identified during this study, of which 52 are epiphytic or lithophytic and 33 are terrestrial orchids. This study identified 57 species or 64.8% were the same as those recorded by Curtis (1894), and 78 species or 66.1% of Turner's (Gardens' Bull Singap 47(2):599-620, 1995) checklist of 118 species for the state of Penang including 18 species which were not recorded by Curtis (1894) and the current study but are actually collected from Penang Hill. A comparison table of the current findings against Curtis (1894) and Turner (1995) is provided which shows only 56 species were the same in all three studies. The preferred account for comparison was Curtis' (1894) list as his report was specifically for the areas around Penang Island especially Penang Hill, Georgetown and Ayer Itam areas. Our study reveals that about 50% of Curtis' collection localities have been converted to residential areas and agricultural land, and this probably explains the decreasing numbers of species found in the current study especially for the terrestrial species as epiphytic species have better adaptation capabilities towards environmental

Corybas is an elusive terrestrial orchid genus mostly inhabiting the high peaks in Peninsular Malaysia, which is the most vulnerable habitat to climate change. Nine species, of which seven are endemic, were studied based on natural populations, whereas information on another two was obtained from herbarium collections. Corybas caudatus was excluded from the study due to insufficient materials, and the type was not located. The species investigated were growing in small populations in the remaining moss-covered forests in the highlands. Human activities and temperature rise are two major threats to the wild populations. Our field observations revealed that Corybas species are good indicators of climate change as they are highly sensitive to temperature rise and drop in relative humidity. Judging from the current small number of specimens recollected, the small population sizes and degraded habitats where they grow, Corybas species in Peninsular Malaysia are all threatened by extinction in the wild.