Study of the genetic diversity in plant inherited stores in order to classify the germplasm regarding resistance to biotic and abiotic stress and also preventing from genetic erosion is one of the basic and fundamental steps in the most breeding programs .Iran is one of the most important centers of genetic diversity due to having different climates and the old civilization. In order to distinguish and evaluate different genetic of aegilops germplasm ,which were collected from west part of the Iran, 80 ecotypes of aegilops by RAPD molecular marker were assessed .DNA extraction was done by modified CTAB method. After DNA extraction stages, complement gene locuses were amplified by 18 RAPD primers. These primers produced 183 bands, that 151 bands (about 82%) were polymorphic. Cluster analysis based on the resulting data was performed using UPGMA method and Dice's similarity coefficient in NTSYS software. The resulting dendogram categorized the accessions into 8 groups in 69% similarity. Principle Component Analysis (PCA) was performed too, 2 and 3 dimensions graphs using 18 primers were drawn.