This study has been done in order to investigate the phytochemicals constitution and determine in vitro antimicrobial activity of aqueous and methanolic Citrus aurantium L.leaves extract against bacteria. The organic solvent extraction done by used Soxhlet apparatus, solvents used were methanol (80%), while aqueous extract was prepared by using magnetic stirrer. The phytochemical analysis of plant extract was performed using precipitation and coloration methods. Antibacterial activity of methanolic and aqueous of Citrus aurantium L. leaves extract determined by disc diffusion and agar well diffusion