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SUMMARYA PCR-based method of detecting Haemophilus influenzae in cultures inoculated from throat swabs was evaluated using samples from groups of laboratory staff and medical students and then applied to samples originating from the closed human community of an Antarctic research station. Suitable PCR primers to an H. influenzae gene (ompP2) were used to amplify the gene from DNA preparations made from mixed growth on chocolate agar with added vancomycin. PCR product was reamplified and subjected to restriction endonuclease digestion to allow temporal and spatial mapping of strains over an 8-month period. Eleven different strains ofH. inftuenzae were detected. One particular strain was detected in a third of the base members.

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Plant tissue culture has revolutionized the field of plant biotechnology. However, there are certain obstacles which overall restrain the output of the plant tissue culturing. One of them is contamination of the tissue culture stock which is a major problem limiting the output. Aegle marmelos (L.) is a medicinal plant whose genotype qualities are maintained through clonal propagation of nodal segment as an explant. It harbors plethora of fungi which curbs the successful in vitro propagation. Chemical fungicide like bavistin is used to prevent the contamination in tissue culture which raises the environmental concerns. Thus, use of microbially derived antifungals can help in preventing fungal growth with benefit of positively impacting the plant growth. Here, authors investigated the use of heat stable lipopeptides which are secondary metabolites derived from Bacillus amyloliquefaciens AB30a for prevention of contamination in tissue culturing of nodal explants of A. marmelos positively impacting its in-vitro propagation. Plant Tissue Cult. & Biotech. 29(1): 111-119, 2019 (June)