Eggs from a F 1 cultured broodstock of sole were compared with eggs from wild-caught breeders throughout one spawning season, to evaluate if egg quality may be affected by culture-related conditions. Fourteen batches of eggs from cultured broodstock and 17 batches from wild-caught sole were compared with respect to fatty acid (FA) composition, egg size, fertilization rate and hatching rate. Based on a multivariate analysis of the FA profiles, it was possible to discriminate between culture and wild inheritance. Eggs from cultured broodstock had high levels of C20:1(n-9), C18:2(n-6) and C18:3(n-3), whereas eggs from wild fish had high levels of C16:1(n-7), C20:4(n-6) and C20:5(n-3). Differences in FA profiles were most likely related to dietary differences. Fertilization and hatching rates were generally low and lowest in eggs from cultured broodstock, but not related to FA composition. Larval growth of one batch from each group was compared. Larval growth was not correlated to broodstock origin, FA composition or egg or larval size. However, larval survival was significantly lower for larvae from cultured broodstock. KEY WORDS