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Chemical data on the lichen Usnea baileyi are scarce. The current investigation reports the extraction, isolation and characterization of depsidones from this lichen collected in Lam Dong Province. Eight depsidones were isolated and elucidated, including methylstictic acid (1), stictic acid (2), cryptostictic acid (3), 8’-O-methylmenegazziaic acid (4), menegazziaic acid (5), 8’- O-methylconstictic acid (6), constictic acid (7), and hypoconstictic acid (8). Multi chromatographic methods comprising normal phase silica gel column chromatography and thin layer chromatography were applied to the crude acetone extract to isolate and purify compounds 1-8. Their chemical structures were elucidated by spectroscopic analysis as well as comparing their data with ones in the literature.

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