18 flowers of Compositae family were collected and extracted in aqueous methanol (MeOH). The concentrated extract was partitioned into n-hexane, ethyl acetate (EtOAc), n-BuOH, and water fractions. The extract and fractions were evaluated for total phenolics, total flavonoids, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and tyrosinase inhibition activity. n-Hexane and EtOAc fractions of Aster yomena, n-

Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl SiO 2 and silica gel (SiO 2 ) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as (3β,5α,8α,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-β-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.

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The roots of Scrophularia buergeriana were extracted with 80% aqueous Methanol and the concentrates were partitioned into EtOAc, n-BuOH, and H 2 O fractions. The repeated silica gel or octadecyl SiO 2 column, and medium pressure liquid chromatographies for the n-BuOH fraction led to isolation of phenylethanoid glycosides and iridoid glycosides. The chemical structures of these compounds were determined as harpagoside (1), angoroside C (2), aucubin (3) and acetoside (4) based on spectroscopic analyses including nuclear magnetic resonance and MS. A simple and efficient HPLC with UV detection method for the simultaneous determination of the four compounds (1-4) has been developed and applied to their content determination in the S. buergeriana. The roots were extracted by 80% methanol, and the contents of 1, 2, 3, and 4 were determined to 11.5, 7.6, 41.2, and 4.8 mg/g, respectively. Additionally, angoroside C (2) and acetoside (4) exhibited hepatoprotective effect against ethanol-induced hepatotoxicity in HepG2 cell line. KeywordsHepG2 · Iridoid · Phenylethanoid · Quantitative analysis · Scrophularia buergeriana 서 론 최근 식생활의 서구화, 알코올 섭취량의 증가 및 음주연령의 낮 아짐 등의 원인으로 간질환 환자가 증가하고 있다[1]. 정상적인 간은 중성지방(triglyceride)을 3-5%를 함유하고 있지만, 지방간 은 지질대사의 장애로 간 조직에 중성지방이 5% 이상 초과하 여 축적된 것으로 정의한다. 지방간은 알코올 이외에도 비만, 당뇨 등의 다양한 원인으로 발생되며 알코올성 지방간(alcoholic fatty liver disease; AFLD)과 비 알코올성 지방간(non-alcoholic fatty liver disease; NAFLD)으로 구분된다[2]. 일반적으로, 알코