We identified a new genotype of bufavirus, BuV3, in fecal samples (0.8%) collected to determine the etiology of diarrhea in children in Bhutan. Norovirus GII.6 was detected in 1 sample; no other viral diarrheal pathogens were detected, suggesting BuV3 as a cause of diarrhea. This study investigates genetic diversity of circulating BuVs.

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A prospective study was performed to determine the molecular characteristics of rotaviruses circulating among children aged <5 years in Bhutan. Stool samples were collected from February 2010 through January 2011 from children who attended two tertiary care hospitals in the capital Thimphu and the eastern regional headquarters, Mongar. The samples positive for rotavirus was mainly comprised genotype G1, followed by G12 and G9. The VP7 and VP4 genes of all genotypes clustered mainly with those of neighboring countries, thereby indicating that they shared common ancestral strains. The VP7 gene of Bhutanese G1 strains belonged to lineage 1c, which differed from the lineages of vaccine strains. Mutations were also identified in the VP7 gene of G1 strains, which may be responsible for neutralization escape strains. Furthermore, we found that lineage 4 of P[8] genotype differed antigenically from the vaccine strains, and mutations were identified in Bhutanese strains of lineage 3. The distribution of rotavirus genotypes varies among years, therefore further research is required to determine the distribution of rotavirus strain genotypes in Bhutan.

Summary Bhutan lies at altitudes of 100–7500 m on the steep, long and complex southern slopes of the Eastern Himalayas. Soil surveys show that, despite steep gradients, there are many moderately or deeply weathered soils. Many slopes are mantled with polycyclic, layered drift materials, so soil horizons owe as much to regolith heterogeneity as to pedogenesis. In the limited arable areas soil profiles are further complicated by rice cultivation and the construction, maintenance and irrigation of flat terraces on steep slopes. Some natural pedogenic horizonation is apparent, and there is an altitudinal zonation of soil types. Although the climate is warm and seasonally wet, most soils on the subtropical southern foothills are not particularly weathered and leached. The foothills are seismically active, and many soils are formed in unstable landslide debris. Elsewhere the regoliths are more stable. The main soils up to about 3000 m in the inner valleys are moderately weathered and leached, and have bright subsoil colours and thin dark topsoils. Above these there is a zone of bright orange‐coloured non‐volcanic andosolic soils. Further upslope there are acid soils with thick surface litter, stagnogleyic topsoils, and drab brown subsoils with organic cutans. These grade to weak podzols, which extend from about 3500 m up to the treeline, around 4000 m. Above this, alpine turf soils, with deep, dark, and friable topsoils and yellowish friable subsoils, are intermixed with unweathered glacial deposits. The interactions between pedogenesis and the deposition of the varied and layered drift materials complicate mapping and classification of the soils.

In addition to the eight genotypes of classic human astroviruses, seven new genotypes have been reported from two novel clades, MLB and VA. However, the epidemiology of these highly diverse astroviruses remains largely unknown. We report here the complete genome sequences of two MLB1 strains from Bhutanese children with diarrhea.