In the last some years, the use of herbal drugs has been increased all over the world due to their huge therapeutic effect and less adverse effects as compared to other medicines. The rising use of herbal drug by the human is forcing the driving force to evaluate the health claim of these agents and to develop standards of quality, purity, safety and efficacy of the drug. Mostly herbal drugs are effective but due to adulteration and lack of standardization, the effectiveness of the herbal drug is decreased. So there is need of development of standardization parameters. In the standardization of the herbal drug the physical, chemical, biological, analytical parameters are carried out. It assures the quality, purity and safety of herbal drug.

Background The purpose of this study was to use the MTT test to assess the cytotoxic effects of different extracts of Convolvulus pluricaulis leaves in vitro. Convolvulus pluricaulis leaf ethanolic extract has been demonstrated to trigger apoptosis on HepG2 cancer cell lines, implying anti-cancer activity. Methods The cells were grown in culture DMEM and incubated with different concentrations of the plant extracts. Survival rates were quantified by MTT assays by 24 h of exposure to (640–20 µg/ml) the PEE, CHCl3E, EAE, ETHE and AQE of the plant, while monitoring changes on cellular shapes by inverted phase contrast microscopy (PEE—petroleum ether extract, CHCl3—chloroform extract, EAE—ethyl acetate extract, ETHE—ethanol extract, AQE—aqueous extract). Results The Convolvulus pluricaulis leaves extract showed IC50 value < 1000 μg/ml on HepG2 and IC50 value > 1000 µg/ml on L-929 cell lines. Hence, Convolvulus pluricaulis leaves extracts are non-toxic against the normal cell line L-929. Treatments with standard as a control exhibited necrotic features in both cell lines. On the basis of these findings, and because a highly effective extract ETHE has a partial polarity, this plant cytotoxicity and apoptotic activities were also investigated. On HepG2 cell line, the ETHE showed higher cytotoxicity activities compared to AQE, EAE, CHCl3E, and PEE extracts (P < 0.0001) with inhibitory concentration IC50 values of 35.873 μg/ml that is < 1000 μg/ml. The percentage of apoptotic cells of ETHE was determined using propidium iodide (PI) staining of DNA fragments by flow cytometry. Conclusions The extract of Convolvulus pluricaulis has cytotoxic and apoptotic action against the HepG2 cell line, indicating that it should be studied further for cancer therapies.

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Objectives: To decide the phytochemical constituents, antioxidant and anticancer potential of Asparagus aethiopicus L. extracts on HT-29 human colon malignant growth cell line. Methods: The roots of plant were exposed to Hot Soxhlet continuous extraction with expanding polarity of solvents viz., pet ether, chloroform, ethanol and aqueous maceration. Qualitative phytochemical screening was completed by utilizing distinctive phytochemical tests. The antioxidant potential was tried utilizing 2, 2-diphenyl-1picrylhydrazyl, ferric ion reducing power assay and phosphomolybdenum assay. In vitro anticancer action tried on HT-29 human colon malignant growth cell line and it was assessed by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT test. Results and Discussion: Preliminary qualitative phytochemical screening affirmed the presence of phytoconstituents like alkaloids, flavonoids, glycosides, saponins, sterols, tannins and reducing sugar. Antioxidant potential was demonstrated most noteworthy in ethanol extracts dependent on the test performed. The ethanol extracts were seen as an antioxidant and specifically cytotoxic to HT-29 human colon malignant growth cell line. Conclusion: The outcomes show that Asparagus aethiopicus L. having a potential of antioxidant activity and anticancer action on HT-29 human colon cancer cell line. In any case, further examinations are expected to presume that the particular constituent liable for its antioxidant action and cancer prevention agent.

In the developing world the trend has been changed from synthetic to natural medicine. Trees and plants are sources of many medicines. The use of medicinal plants was compiled in Ayurveda which listed more than 8000 herbal remedies. Mimosa rubicaulis Lam belonging to the family Fabaceae and subfamily Mimosoideae. It is bipinnately compound, each leaf having 8–12 pairs of pinnae, each with 16–20 pairs of pinnules, unlike this plant is having good potential activity in plant due to it has been attributed to their flavanoid and sterol contents. Several flavanoids isolated from these plants have been discovered to possess significant Various Pharmacological activity. Literature review reports the presence of flavanoids, triterpenoids, phytosterols and alkaloids in Mimosa rubicaulis Lam.