This study was conducted to investigate the nature of colonic metaplasia in ileo-anal pouches and incidence / frequency of pouchitis in the same. Biopsy specimens from 8 patients with functioning ileal pouches were studied using routine histology, mucin histochemistry and electron microscopy, over a 2 -year period. All 8 patients had villous abnormalities in the form of blunting of villi and sub total or partial villous atrophy. 6 patients had an increase in the goblet cell population and Paneth cell hyperplasia. These changes were supported by electron microscopic findings of a decrease in number and flattening of ileal type microvilli and their transformed morphologic resemblance to colonic type microvilli. All the ileal pouches also had acquired colorectal type sulphomucin, when sections stained with Alcian-blue and High Iron Diamine -Alcian blue, were studied. However, no case of pouchitis as defined in literature, was found in this study.
MJAFI 2003; 59 : 306-309
Basal cell hyperplasia, a common finding in benign prostatic hyperplasia, refers to proliferation of basal/ reserve cells. However, occasionally it is so florid and extensive that it can be mistaken for carcinoma. Accurate distinction separating benign and malignant lesions is critical for appropriate management. We report two elderly gentlemen who presented with signs and symptoms of prostatomegaly, histology showed florid basal cell hyperplasia with pseudo-infiltrative pattern raising a doubt of carcinoma warranting a second opinion. Review of histopathology with multiplex immunohistochemistry panel combining basal cell, metabolic, proliferation and other markers proved the benign nature of the lesion. Thereby, we conclude that although morphologic criteria guide diagnosis, immunohistochemistry confirms the benign nature resolving the dilemma even on limited material.
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