This study aimed to evaluate the potential of Girolando (Bos taurus × Bos indicus) cows with high and low antral follicle counts (AFC) for the in vitro production of bovine embryos (IVEP), as well as the pregnancy rates of the recipients of these embryos. Girolando cows (Bos taurus × Bos indicus) were classified as high and low AFC when they had 35-52 (n = 13) and 11-17 follicles (n = 15), respectively. All animals were subjected to repeated follicular aspiration [Ovum pick-up (OPU)] and subsequent IVEP sessions. The synchronization protocol of the recipients was performed on a random day of the estrous cycle (Day 0) with the implantation of progesterone, estradiol benzoate, and prostaglandin. The high AFC group had higher aspirated oocyte/OPU (42.6 ± 5.2 vs. 14.6 ± 1.9; p < 0.01) and cultured oocyte/OPU (38.1 ± 6.6 vs. 12.3 ± 2.8; p < 0.01) averages as well as a higher blastocyst percentage on D7 (23.0 ± 1.0% vs. 18.4 ± 1.5%; p < 0.05) and higher pregnancy rate (42.7 ± 2.7% vs. 39.7 ± 4.6%; p < 0.05) than the low AFC group. Thus, we can conclude that animals with high AFC had better IVEP success rates than animals with low AFC.
O objetivo da pesquisa que origina este trabalho foi avaliar a influência da adição de antioxidantes ao meio de maturação sobre a maturação nuclear in vitro de oócitos bovinos obtidos de abatedouro. Para sua execução foram utilizados somente ovários de fêmeas bovinas zebuínas. Os folículos foram aspirados e os oócitos recuperados, selecionados e classificados. Utilizaram-se três meios (TCM 199 com Antioxidant Supplement [sigma A-1345], TCM 199 suplementado com ácido ascórbico e TCM 199 suplementado com glutamina); para avaliar a maturação nuclear. Não foi possível verificar a ação protetora ou anti-oxidativa dos tratamentos, pois não houve diferença significativa dos diferentes estádios de maturação. Sugere-se dar continuidade aos experimentos, com objetivo de aumentar o n Total, para que se possa verificar novamente a existência de possíveis diferenças entre os meios propostos. Palavras-chave: Maturação in vitro. Bos indicus. Antioxidante. Ácido ascórbico. Glutamina.
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