Introduction:The present study focuses on the use of Leucas aspera aqueous extract as a reduction agent for the synthesis of copper nanoparticles (CuNPs). The entire plant is employed in conventional medicine and as an insecticide. Scientific research has been done on the plant's potential as an anti-inflammatory, analgesic, and wound-healing agent. Intoxication by cobra venom also makes use of it. This study was intended to explore the in vitro anti-inflammatory, antidiabetic and anticancer activities of copper nanoparticles. Methods: Protein denaturation and membrane stabilization of human red blood cells (HRBC) were used to assess the in vitro anti-inflammatory activity. The antidiabetic activity of the CuNPs was evaluated by inhibiting both amylase and glucosidase in vitro, whereas anticancer activity against vero and MCF-7 cell line. Results: The Leucas aspera copper nanoparticles (LACuNPs) showed a dose dependent -inflammatory activity. The highest inhibition percentage in protein denaturation is 70.30±1.72% and 67.68±1.41% in HRBC membrane stabilization at 1000µg/ml concentration. In vitro antidiabetic activity exhibited 76.03 ± 1.14 of α-amylase and 71.54 ± 1.55 at α-glucosidase inhibition assay. The in vitro anti-cancer study revealed 50% apoptosis (IC 50 ) of LACuNPs treated vero and MCF7 are displayed as 248.84±0.01 µg/ml and 137.39±1.51, respectively. Conclusion: The results clearly demonstrated the application of biosynthesized LACuNPs as potential anti-inflammatory, anti-diabetic, and anticancer agents in addition to their environmental friendliness.

Introduction: Leaves of Tephrosia villosa belonging to the family fabaceae being used for the treatment of dropsy and diabetes. The present research work was under taken to investigate the in-vitro antioxidant activity of petroleum ether, chloroform, ethyl acetate, ethanol, methanol, and water extracts. Methods: In vitro antioxidant activities were carried out for six solvent extracts such as T. villosa leaf petroleum ether extract, T. villosa leaf chloroform extract, T. villosa leaf ethylacetate extract, T. villosa leaf ethanol extract, T. villosa leaf methanol extract, and T. villosa leaf water extract by standard methods. Five various antioxidant methods such as DPPH radical scavenging ability, superoxide radical scavenging potential, ABTS scavenging capacity, metal chelating ability, and reducing power assay (FRAP) were estimated. Results: Further investigation was carried out for in-vitro antioxidant activity and radical scavenging assay by calculating its % inhibition by means of IC50 values, all the extracts concentration has been adjusted to come under the linearity range and here reference standards ascorbic acid has been taken for the method suitability. Conclusion: The results revealed that leaves of this plant have antioxidant potential. Among these results, methenolic and water extract has more potent than the other extract. T. villosa Linn. (Fabaceae) leaves possess the antioxidant substance which may be potential responsible for the treatment of oxidative stress related diseases.