To develop vaccine for rohu (Labeo rohita), efficacy of three antigenic preparations from Aeromonas hydrophila were evaluated. Thirty six tanks with ten rohu were divided in quadruplicates (R1 to R4) with nine tanks (G1 to G9). Rohu of G1 to G6 tanks were given intraperitoneal vaccine with outer membrane protein, somatic protein and formalin-inactivated whole cell itself and along with Incomplete Freund's Adjuvant @ 200 µg/fish, G7 and G8 tanks were injected with Incomplete Freund's Adjuvant (100 µl/fish) and normal saline (100 µl/fish) respectively and G9 tanks were kept as control. After 28 d, rohu of R3 and R4 were subjected to intramuscular A. hydrophila challenge (LD50) @ 2.85×10 6 cells/fish for 7 d and RPS (%) was calculated. Specific cellular and humoral immune responses were determined for rohu of R1 and R2. Results showed that rohu immunized with outer membrane protein along with adjuvant could offer an appropriate vaccine strategy.
The study was carried out to find the prevalence and severity of Argulus sp. in Indian Major Carps (IMC) collected from Bhangore block of South 24 Parganas district of West Bengal during November’2018 to August’2019. The highest parasitic prevalence (PFI, 83.33%) had been recorded in the month of January and lowest (PFI, 9%) in the month of August. The identities of selected parasites were further confirmed by molecular identification through 18S rDNA analysis. The study revealed that Argulus infestation was of great implications from economic point of view throughout the study period especially in winter and is the most prevalent problem in fresh water aquaculture systems.
The study was carried out to find the prevalence of ectoparasites in carp species specifically Indian Major Carps (IMC) during the post monsoon season (November’16 to March’17). Four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from approximately 500 number of carp species like, Labeo rohita, Catla catla, Cirrhinus mrigala and Labeo calbasu collected from different ponds of selected blocks of South 24 Parganas district of West Bengal. The highest prevalence (64.76%) of infestation had been recorded by Myxozoans and the lowest was by Monogeneans (4.79%). The highest and lowest ectoparasitic prevalence in carp was observed in L. rohita (32.88%) and C. catla (27.28%). At the end of this experiment it was concluded that sudden changes in water quality parameters, over stocking, lack of management practices and some other aspects were the key factors for disease occurrences in this post monsoon season.
The study was carried out to find the prevalence of ectoparasites in carp species specifically Indian Major Carps (IMC) during the post monsoon season (November’16 to March’17). Four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from a total 500 number of carp species like, Labeo rohita, Catla catla, Cirrhinus mrigala and Labeo calbasu collected from different ponds of selected blocks of South 24-Parganas district of West Bengal. The highest prevalence (64.8%) of infestation had been recorded by Myxozoans and the lowest was by Monogeneans (4.8%). The highest and lowest ectoparasitic prevalence in carp was observed in L. rohita (32.9%) and C. catla (27.3%). Beside these, lower temperature (Average 19.3oC), low pH (Average 6.9) and marginal level of dissolved oxygen (Average 6.0ppm) were also created an unfavorable condition for parasitic infestation during this season. At the end of this experiment it was concluded that disease occurrences due to ectoparasites was high in winter with some key factors like temperature, pH and dissolved oxygen (DO).
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