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A novel preparation method for surfactantHowever, to take full advantage of the biocatalysis lipase complexes has been developed utilizing water in in an organic medium, it is necessary to modify the oil emulsions. In order to optimize the preparation condienzymes to be soluble in an organic solvent from the tions, we have investigated the effects of several operaviewpoint of reduced diffusional limitations of subtional parameters on the enzymatic activity of the surfacstrates and increase in reaction rates. coated with surfactants through a noncovalent interac- 455-460, 1997. tion and the coated enzymes are almost entirely soluble

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