BackgroundThis study aims to characterize the housekeeping and tissue-specific genes in 15 mouse tissues by using the serial analysis of gene expression (SAGE) strategy which indicates the relative level of expression for each transcript matched to the tag.ResultsHere, we identified constantly expressed housekeeping genes, such as eukaryotic translation elongation factor 2, which is expressed in all tissues without significant difference in expression levels. Moreover, most of these genes were not regulated by experimental conditions such as steroid hormones, adrenalectomy and gonadectomy. In addition, we report previously postulated housekeeping genes such as peptidyl-prolyl cis-trans isomerase A, glyceraldehyde-3-phosphate dehydrogenase and beta-actin, which are expressed in all the tissues, but with significant difference in their expression levels. We have also identified genes uniquely detected in each of the 15 tissues and other tissues from public databases.ConclusionThese identified housekeeping genes could represent appropriate controls for RT-PCR and northern blot when comparing the expression levels of genes in several tissues. The results reveal several tissue-specific genes highly expressed in testis and pituitary gland. Furthermore, the main function of tissue-specific genes expressed in liver, lung and bone is the cell defence, whereas several keratins involved in cell structure function are exclusively detected in skin and vagina. The results from this study can be used for example to target a tissue for agent delivering by using the promoter of tissue-specific genes. Moreover, this study could be used as basis for further researches on physiology and pathology of these tissues.

This study characterizes the most highly expressed transcripts of 15 intact tissues in mice by using the serial analysis of gene expression (SAGE) strategy which indicates the relative level of expression for each transcript matched to the tag. We show that the most abundant transcripts in the prostate, testis, and skeletal muscle characterize the main functions of these organs as an exocrine gland of male reproduction, spermatogenesis, and contraction, respectively. In addition, the top nine most abundant transcripts in the testis are tissue-specific genes while the most abundant transcripts in the prostate are also abundantly expressed in the liver. Furthermore, the most abundant transcripts in the ovary, mammary gland, and vagina are related to steroidogenesis, adipocytes, and keratinization, respectively, whereas genes involved in the cell defence are abundantly expressed in the liver, lung, bone, mammary gland, and adipose tissue. These findings suggest that the top 10 transcripts are sufficient to characterize each tissue of the body.