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The synthesis and characterization of an aggregate of AgNPs coated with plant extract (PE) from and its significant antimicrobial activity and inhibition on K562 (blood cancer) cells have been appended in the article. Synthesis of aggregate [(AgNPs)-(PE)] has been followed by a facile eco-friendly approach without using any harmful chemicals. The morphology of an aggregate [(AgNPs)-(PE)] was confirmed by TEM and SEM microscopic characterizations. Properties like solid state, the presence of functional groups, and elemental composition have been characterized through the XRD, FTIR, and EDAX. The biocompatibility of synthesized aggregate of [(AgNPs)-(PE)] was confirmed by the MTT assay. An in vitro cell (HEK293)-based studies were performed for the biocompatibility tests and it is found that the aggregate [(AgNPs)-(PE)] is not harmful to normal/healthy cells. Even though show the antimicrobial (antibacterial and antifungal) activity, it has been further enhanced with the developed aggregate of [(AgNPs)-(PE)]. Furthermore, it has been extended to examine the cellular inhibition on K562 cells and obtained > 75% cell inhibition for 24 h treated cells.

Neptunia oleracea Lour (water mimosa) is an edible medicinal plant used in treating various diseases. According to Phytochemical and Ethnobotanical Databases, Neptunia oleracea Lour is used in curing earaches, dysentery, syphilis, and tumour. The present study was aimed at demonstrating the anticancer activity of the Neptunia oleracea Lour methanolic extract. The methanolic extract was isolated and its anti-proliferative activity was studied on haematological cancer cell lines. The activity of the extract was further evaluated using cell cycle analysis and apoptosis assays. The effect of the extract on c-Myc and PErk1/2 modulation was also evaluated.Neptunia oleracea Lour extract induced cell death in cancer cells while sparing normal cells. An increase in cleaved PARP and reduction in BCL-2 levels observed upon treatment. Neptunia oleracea causes reduction in c-Myc levels and pERK1/2 protein levels. Thus, our work highlights the methanolic extract of Neptunia oleracea Lour as a promising anti-cancer agent.

Thermophilic fungi have often been proposed as magnificent sources, since, they grow in extremely harsh temperatures, ranging between 20-50 °C. They have ability to produce industrially germane thermo-resistant enzymes that can endure the austere environment of industrialized procedures, and have outspread mercantile acceptability in food, paper, feed, pulp and also in textile industries. The isolates of numerous thermophilic fungi are sources of biotechnologically valuable enzymes like pectinases, laccases, xylanases, phytases, proteases and cellulases etc., which display high levels of temperature tolerances up to a maximum range of 72 °C. Despite lesser-known knowledge about them, they have already aided humans in many ways and still have lot more to give. In the subsequent years, the utilization of thermostable enzymes is anticipated to escalate exponentially due to their role in biofuels. In this review I describe about their isolation from their respective habitat, production of thermostable enzymes and their applications in industry, which helped scientists to break through the field of Industrial Biotechnology.