Alkali tetraborate glasses 10RO-30ZnO-xLi2B4O7-(60-x) K2B4O7 (R=Mg, Ca) with x varying from 0 to 60 mole% were synthesized by melt quenching approach at around 1150°C. Broad and peak less X-ray diffraction spectra confirmed the amorphous nature of synthesized glass samples. Physical and optical properties, namely density, molar volume, refractive index, optical band gaps, molar refractivity, and Urbach energy values, have been reported. The density of the present glass samples improved with increasing x mole% except at the presence of equal amounts of alkali oxides in the glass system. The molar volume decreased continuously with x mole%. Oxygen packing density increased with increasing x mole%. The optical energy band gap Eopt values with increasing lithium tetraborate in the glass composition have shown a downward trend, where are refractive index values shown upward. Fourier transform infrared and Raman studies revealed the variation of BO3 and BO4 units in the glasses with composition is marginal. EPR spectra of copper ions confirmed the existence of Cu2+ ions in the ground state dx2-y2 orbital (2B1g). The observed non-linear variations of various properties are attributed to the structural changes caused by mixed alkali effect.