The aim of work is to extract and characterize the Vigna mungo polymer solvent using acetone and ethanol. Natural polymers contribution towards formulation of dosage forms is appreciable as they are biocompatible, biodegradable and safe. So extraction and characterization of Vigna mungo polymer helps in the interaction studies of preformulation. In this present study, various physicochemical characters like phytochemical screening, viscosity, particle size analysis, and flow characteristics were determined. Further characterization performed using FTIR and XRD. Vigna mungo polymer obtained using acetone was taken into further studies of evaluation because of more product yield and less particle size. FTIR results revealed existence of carbohydrate nature. X-ray diffractogram presented degree of crystallinity 26.4%. And phytochemical screening of the extracted polymer indicated presence of mucilage and carbohydrates using ruthenium red and molisch's test. Statistical analysis of data was performed using two way ANOVA using Graphpad prism 5 software was used to compare Vigna mungo polymer extracted using acetone and ethanol. Physicochemical parameters experimental data found to be statistical significance two way ANOVA (P < 0.05).

The aim of the present research is to investigate acute toxicity profiling of isolated Vigna mungo new natural polymer. Safety administration is the primitive criterion for any drug substance. To explore the safety and toxicity profiling of the novel polymer, this study was carried out. Vigna mungo novel polymer was isolated from the pulverised seeds of Vigna mungo which is part and parcel of our diet. This polymer is obtained using a non-solvent extraction method using acetone. Acute toxicity studies were performed according to the OECD guidelines 420. In this, the selected animal model is Swiss albino rats, grouped into control and test containing each three animals. 2000 mg/kg of Vigna mungo polymer was administered to a test group and did not produced any abnormalities and behavioural changes. Furthermore, histopathological studies, body weight, haematological parameters did not presented abnormal values. The observations found 2000mg/kg of a dose of the polymer did not cause lethality and death of any animal till 14 days of a period. It was concluded that Vigna mungo novel polymer is safe to administer up to 2000mg/kg dose. Hence, the novel Vigna mungo polymer is safer for therapeutic use in pharmaceutical formulations.

In this study bioadhesive sustained release tablets of metronidazole for once daily administration were formulated. This formulation helps to increase the localized effect of metronidazole by formulating the vaginal bioadhesive tablet and to increase the ease of application when compared to various types of vaginal gels and creams that are available in the market.Metronidazole is a nitro imidazole derivative class of anti-protozoal drug used to treat amoebiasis, vaginitis, trichomonal infections, trepenomal infections and giardisis. The objective of the present study is to formulate the bioadhesive controlled release drug delivery system which would remain in contact with the vaginal tissue for prolonged period of time in view to maximize the bioavailability and therapeutic efficacy of the drug.Bioadhesive tablets were prepared using metronidazole and sodium alginate in different proportions by wet granulation method. The prepared tablets were evaluated for weight variation, hardness, friability, dissolution and swelling studies.The release of drug from various vaginal bioadhesive tablets exhibited the following order F4>F1>F3, but F2 exhibited faster drug release compared to other formulations, which is not a desired characteristic for the treatment of vaginosis. By observing the above results, more ca +2 ions became available to bind with sodium alginate during the wet granulation stage of the preparation. As a result better and stronger gel was formed when high amount of calcium carbonate was used. As the concentration of ca +2 ions increases, stronger gel of calcium alginate is formed that delay the influx of the dissolution medium and efflux of the dissolved drug out the matrix. As a result drug is released in amore sustained manner.

The main objective of the present research  study was to fabricate Vildagliptin  floating microspheres  by ionotropic gelation using natural polymer, Abelmoschus esculentus obtained from the fruits of Abelmoschus esculentus in combination with sodium alginate. Microspheres were prepared and optimized using central composite rotatable design model using design expert software  version  12  .The  study  is  focussed  on  the  interaction  effects  of  the  three independent  variables,  natural  polymer concentration, sodium alginate concentration and crosslinker concentration, optimization of formulations   response surface methodology  was used  . drug –excipient  compatibility studies  were carried out  by infrared spectroscopic studies. Infrared spectroscopic studies clearly shown that drug and excipients were compatible. Totally 15 formulations were generated taking 8 factorial points, 6 axial points and 1 centre point. Response surface methodology   was used to optimize the formulations. To investigate the responses %cumulative drug release, floating time and floating lag time Response surface methodology was used Polynomial equations and model plots of 3 dimensional model surface plots were generated.  Vildagliptin optimized microspheres were formulated and a second order, model   quadratic model was used to study influence of formulation factors on response variables. Experimental data of Statistical analysis exhibited good coefficient of regression for cumulative in vitro drug release Regression  F –ratios for the experimental  variables were significant. The experimental  values and predicted  values are agreed. All fifteen formulations  exhibited  % yield of 94.35-99.99%,  particle  size of 124-441µm,  %swelling  index 64.52-89.65%,  floating time of 10.16 to 13.16 and floating lag time of 30.31 to 46.98 sec. F4 formulation  is optimized based on cumulative  % in-vitro drug release at 2nd  hour, 12th  hour, floating lag time and floating time values. Predicted and observed results are in agreement 95% confidence intervals. Based on investigation, RSM is the good tool for optimization of formulations.