Vitamin D deficiency is associated with osteoporosis and is thought to increase the risk of cancer and CVD. Despite these numerous potential health effects, data on vitamin D status at the population level and within key subgroups are limited. The aims of the present study were to examine patterns of 25-hydroxyvitamin D (25(OH)D) levels worldwide and to assess differences by age, sex and region. In a systematic literature review using the Medline and EMBASE databases, we identified 195 studies conducted in forty-four countries involving more than 168 000 participants. Mean population-level 25(OH)D values varied considerably across the studies (range 4·9-136·2 nmol/l), with 37·3 % of the studies reporting mean values below 50 nmol/l. The highest 25(OH)D values were observed in North America. Although age-related differences were observed in the Asia/Pacific and Middle East/Africa regions, they were not observed elsewhere and sex-related differences were not observed in any region. Substantial heterogeneity between the studies precluded drawing conclusions on overall vitamin D status at the population level. Exploratory analyses, however, suggested that newborns and institutionalised elderly from several regions worldwide appeared to be at a generally higher risk of exhibiting lower 25(OH)D values. Substantial details on worldwide patterns of vitamin D status at the population level and within key subgroups are needed to inform public health policy development to reduce risk for potential health consequences of an inadequate vitamin D status. Key words: Vitamin D: Populations: Public healthVitamin D plays an important role in bone mineralisation and other metabolic processes in the human body such as Ca and phosphate homeostasis and skeletal growth (1,2) . Vitamin D deficiency, for example, causes rickets in children, leading to skeletal abnormalities, short stature, delayed development or failure to thrive (3) . In adults, low values of vitamin D are associated with osteomalacia, osteopenia, osteoporosis and subsequent risk of fractures (1) . In addition to beneficial effects on musculoskeletal health, observational studies have suggested that low 25-hydroxyvitamin D (25(OH)D) values are associated with an increased risk for several extraskeletal diseases including cancer, infections, autoimmune diseases and CVD (4) . In light of the global ageing population (5) ,an almost fourfold increase in osteoporotic hip fractures since 1990 (6) and the possible risk of other chronic diseases, patterns of low 25(OH)D levels are of substantial public health interest. Vitamin D status is traditionally measured through assays of 25(OH)D, the major circulating form of vitamin D (7) . Although 25(OH)D levels below 25 nmol/l have been associated with disorders of bone metabolism (8) and are used to indicate severe vitamin D deficiency, the threshold for defining adequate stores of vitamin D in humans has not been established clearly (9) . The Institute of Medicine has suggested, for example, that approximately 97·5 % of t...

We present a novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers. It is based on transcription factors and we used it to isolate new L-lysine producing mutants of C. glutamicum from a large library of mutagenized cells using fluorescence-activated cell sorting (FACS). This high-throughput method fills the gap between existing high-throughput methods for mutant generation and genome analysis. The technology has diverse applications in the analysis of producer populations and screening of mutant libraries that carry mutations in plasmids or genomes.

This study provides an overview of 25(OH)D levels around the globe. It reveals large gaps in information in children and adolescents and smaller but important gaps in adults. In view of the importance of vitamin D to musculoskeletal growth, development, and preservation, and of its potential importance in other tissues, we strongly encourage new research to clearly define 25(OH)D status around the world.