Electrospun PLCL nanofibrous materials produced by direct current (DC) or alternating current (AC) electrospinning are good candidates for absorbable reinforcement for cardiovascular surgery composite bandages. For an appropriate description of the fiber layers and prediction of its relevant biomechanical characteristics, the knowledge of fiber orientation in the electrospun layers is essential. In this study, the different orientation of fibres in AC and DC electrospun materials was achieved only by changing the rewinding speed of the supporting material, as the easy way for the production of relatively large samples with controlled fiber orientation. The fiber orientation was measured on scanning electron microscope images at different magnification using an open-source image processing software FIJI/Image J with the implementation of the "OrientationJ" plugin. Subsequently, the fiber layers were compared in terms of fiber orientation. Optimal image parameters for the measurement and the best unidirectional oriented-aligned samples were determined
Biodegradable electrospun materials are widely used for medical application. Polycaprolatone is polymer suitable for electrospinning technology and is very often used to create nanofibrous scaffolds for tissue engineering. The time to disintegration or biodegradation of such materials is very important here. However, testing is not entirely easy. It is not possible to create exactly body-like conditions in vitro. Moreover, it is not easy to find suitable analytical methods that would show exactly what happens in the nanofibrous polycaprolactone electrospun samples at certain stages of degradation, ie how the internal structure of decaying nanofibers changes. This paper describes the traditional use of methods for testing polycaprolatone nanofibers by enzymatically catalysed degradation. Morphological changes are studied using scanning electron microscope images. However, it also offers a non-traditional analysis of polycaprolactone electrospun materials using the ssNMR method.
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