The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel: Małgorzata M. DOBRZYŃSKA, et al. Department of Radiation Hygiene and Radiobiology, National Institute of Public Health – National Institute of Hygiene, Poland— Objectives The aim of this study was estimation of DNA strand breaks in leukocytes of peripheral blood of staff in a nuclear medicine department. Methods The exposed group consisted of 46 volunteers and the control group consisted of 40 volunteers. Samples consisting of 1 ml whole blood were collected by venepuncture. DNA damage in leukocytes was detected by alkaline comet assay. Results There was no correlation between the effective dose measured by individual dosimeters and DNA damage and no differences between sexes. The mean level of damage to DNA in people exposed to ionizing radiation was significantly elevated compared with control individuals. The highest value for mean comet tail moment was noted in leukocytes of PET/CT and scintigraphy technicians (1.28 vs. 0.30 for control, p=0.013). The levels of DNA damage in leukocytes of workers in category B (effective dose may exceed 1 mSv/year) were significantly enhanced. The DNA migration of leukocytes in exposed smokers and nonsmokers was similar. In the control group the damage to DNA of leukocytes in smokers was markedly but not significantly higher compared with nonsmokers. Conclusions Occupational exposure to ionizing radiation leads to enhanced levels of reversible DNA damage in leukocytes of nuclear medicine employees. The level of DNA damage depends on the kind of work. Cigarette smoking is related to the increase in DNA damage in unexposed individuals but not in nuclear medicine workers. Radiation seems to be a stronger inducer of DNA damage than smoking. Although most of the DNA damage detected by comet assay is repaired, further improvement of radiation safety should be taken under consideration.

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Bisphenol A (BPA) is employed in the manufacturing of epoxy, polyester-styrene, and polycarbonate resins, which are used for the production of baby and water bottles and reusable containers, food and beverage packing, dental fillings and sealants. The study was designed to examine the effects of 8-week exposure (a full cycle of spermatogenesis) to BPA alone and in a combination with X-irradiation on the reproductive organs and germ cells of adult and pubescent male mice. Pzh:Sfis male mice were exposed to BPA (5, 10, and 20 mg/kg) or X-rays (0.05 Gy) or to a combination of both (0.05 Gy + 5 mg/kg bw BPA). The following parameters were examined: sperm count, sperm motility, sperm morphology, and DNA damage in male gametes. Both BPA and X-rays alone diminished sperm quality. BPA exposure significantly reduced sperm count in pubescent males compared to adult mice, with degenerative changes detected in seminiferous epithelium. This may suggest a higher susceptibility of germ cells of younger males to BPA action. Combined BPA with X-ray treatment enhanced the harmful effect induced by BPA alone in male germ cells of adult males, whereas low-dose irradiation showed sometimes protective or additive effects in pubescent mice.

Background: Due to its use of ionising radiation, the field of nuclear medicine is a unique and significant part of medical diagnostics and patient treatment. The aim of this study was to assess the internal exposure of nuclear medicine employees to radioiodine 131 I and technetium 99m Tc as well as to assess the external exposure doses. Material and Methods: The radioiodine 131 I and technetium

Background: X-ray examination is associated with patient exposure to ionizing radiation. Dose values depend on the type of medical procedure used, the X-ray unit technical condition and exposure conditions selected. The aim of this study was to determine the dose value received by patients during certain conventional radiography X-ray examinations and to assess the technical condition of medical equipment used for this purpose. Material and Methods: The study covered the total number of 118 conventional diagnostic X-ray units located in the Masovian Voivodeship. The methodology used to assess the conventional diagnostic X-ray unit technical condition and the measurement of the radiation dose rate received by patients are based on test procedures developed by the Department of Radiation Protection and Radiobiology of the National Institute of Public Health -National Institute of Hygiene (Warszawa, Poland) accredited for compliance with PN-EN 17025 standard by the Polish Centre for Accreditation. Results: It was found that 84.7% of X-ray units fully meet the criteria set out in the Polish legislation regarding the safe use of ionizing radiation in medicine, while 15.3% of the units do not meet some of them. The broadest dose value range was recorded for adult patients. Particularly, during lateral (LAT) lumbar spine radiography the recorded entrance surface dose (ESD) values ranged from 283.5 to 7827 μGy (mean: 2183.3 μGy). Conclusions: It is absolutely necessary to constantly monitor the technical condition of all X-ray units, because it affects population exposure to ionizing radiation. Furthermore, it is essential to raise radiographers' awareness of the effects that ionizing radiation exposure can have on the human body. Med Pr 2014;65(6):715-721 StreszczenieWstęp: Wykonywanie badań rentgenowskich wiąże się z narażeniem pacjenta na działanie promieniowania jonizującego. Wielkość tego narażenia zależna jest od wykonywanej procedury medycznej, stanu technicznego aparatu rentgenowskiego i dobranych warunków ekspozycji. Celem niniejszych badań było określenie wielkości dawek, na jakie narażeni są pacjenci podczas wybranych ogólnodiagnostycznych badań rentgenowskich oraz ocena stanu technicznego używanej aparatury medycznej. Materiał i metody: Badaniami objęto łącznie 118 ogólnodiagnostycznych aparatów rentgenowskich, zlokalizowanych na terenie województwa mazowieckiego. Metodyka badań dotycząca oceny ich stanu technicznego i pomiaru dawek otrzymywanych przez pacjentów oparta została na opracowanych w Zakładzie Higieny Radiacyjnej i Radiobiologii (Narodowy Instytut Zdrowia Publicznego -Państwowy Zakład Higieny) procedurach badawczych, akredytowanych na zgodność z normą PN-EN 17025 przez Polskie Centrum Akredytacji. Wyniki: W wyniku przeprowadzonych badań stwierdzono, że 84,7% aparatów w pełni spełnia kryteria określone w polskim ustawodawstwie dotyczącym zasad bezpiecznego stosowania promieniowania jonizującego w medycynie, a 15,3% nie spełnia części z nich. Największą rozpiętość otrzymywanych dawek zaobserwowano u ...