Objective: The aim of this study was to isolate and screen for the antimicrobial properties of actinomycetes from marine soil sediments and sponges. Methods: The samples of sponges and sediments were collected from various locations from the coast of Andhra Pradesh. Isolation of actinomycetes was done by plating them on starch casein agar, kuster's agar, Actinomycete isolation agar and potassium tellurite agar medium using dilution technique. Various pathogenic bacterial strains, fungal strains and yeasts were obtained from Microbial Type Culture