Dietary supplementation based on sea buckthorn and rosehip oils with added chokeberry extract was studied. We added the dietary supplement to the feed mixtures for laboratory animals. The possible toxicological effects and hypocholesterolemic, hepatoprotective activity of the dietary supplement in vivo were studied. After the observation period (6 weeks), no significant changes were found in the mass of organs and blood serum of laboratory animals (p > 0.05). However, there was a decrease in hypercholesterolemic indicators. Regular consumption of sea buckthorn and rosehip oils with added chokeberry extract (dietary supplement “ESB-1”) by laboratory animals inhibited the activity of liver enzymes and increased the antioxidant activity of blood serum (after the subcutaneous injection of sunflower oil/oil solution of carbon tetrachloride) but was not sufficient to bring them to physiological standards. The hypocholesterolemic and antioxidant properties of our dietary supplement already allow us to consider it a component of functional food products or a dietary supplement base. However, the full range of its biologically active properties, including the hepatoprotective function and regulation of metabolic disorders, has not been studied yet, which sets the direction of further research in vivo models and clinical practice to confirm its effectiveness in humans.
Authors do researches on the development of new functional ingredients at complex processing of plant raw materials of the Siberian region. As a result of the conducted researches, the choice of plant raw materials components is substantiated (fruits of sea-buckthorn) as sources of functional ingredients. The resource-saving technological scheme of complex processing of fruits of sea-buckthorn where along with sea-buckthorn oil the production of fruit powder from pulp of sea-buckthorn and clarified juice has been offered to develop. Rational use of seeds is provided. Complex dietary supplements based on the products of seabuckthorn processing have been developed. Efficiency of the developed antioxidant and emulsifying additives using natural biologically active agents in the production technology of food emulsions has been theoretically proved and experimentally confirmed. Relevance and prospects of further developments and researches in the field of new domestic micro-ingredients development have been proved.
This article describes the effect of organic acids and ultrasound on the physiological and biochemical properties of yeast, which was used to obtain biologically active peptides. The research featured brewer’s yeast S. cerevisiae W-34/70 cultivated in 11% beer wort. A mix of Krebs cycle acids served as an activator. It included succinic, malic, fumaric, citric, and oxaloacetic acids (1:1:1:1:1). The concentration of the Krebs cycle acids was 1 × 10−10 M/L at 1% to the suspension volume. The ultrasound treatment had an intensity of 10 W/m2 and lasted 3–10 min. The combined effect increased the fermentation activity of the yeast by 98%. The activity of individual biocatalysts of constructive and energy metabolism rose by 108–330%, while that of proteolysis enzymes increased by 15% in comparison with the samples exposed to individual factors. The stimulation increased the rate of amine nitrogen consumption by the yeast. The amount of accumulated amino acids was larger by 80% than in the control, and that of protein larger by 7%. The maximal content of the synthesized protein was reached 1–2 h earlier. The combination of chemical and physical factors intensified the biosynthesis of protein and its intermediates during yeast processing, thus facilitating the subsequent extraction of biologically valuable components.
The article is devoted to the study of the possibility of using natural zeolites as adsorbents for the purification of unrefined vegetable oils, intended for direct consumption in food, from undesirable concomitant substances. To increase their sorption capacity, various activation methods were used, such as physical and chemical. The best sorption properties were found in zeolites, activated by the complex method (heat and acid treatment). Conducted refining of unrefined sunflower oil allowed to increase its quality to meet the requirements of the state standard for the highest grade oil.
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