An image fusion method has been proposed for plant images taken using a two-dimensional (2D) camera and three-dimensional (3D) portable lidar for obtaining a 3D distribution of physiological and biochemical plant properties. In this method, a 2D multispectral camera with five bands (475-840 nm) and a 3D high-resolution portable scanning lidar were applied to three sets of sample trees. After producing vegetation index (VI) images from multispectral images, 3D point cloud lidar data were projected onto the 2D plane based on perspective projection, keeping the depth information of each of the lidar points. The VI images were 2D registered to the lidar projected image based on the projective transformation and VI 3D point cloud images were reconstructed based on the depth information. Based on the relationship between the VI values and chlorophyll contents taken by a soil and plant analysis development (SPAD)-502 plus chlorophyll meter, 3D distribution images of the chlorophyll contents were produced. Similarly, a thermal 3D image for a sample was also produced. The resultant chlorophyll distribution images offered vertical and horizontal distributions, and those for each orientation for each sample, showing the spatial variability of the distribution and the difference between the samples.

It is critical to take the variability of leaf angle distribution into account in a remote sensing analysis of a canopy system. Due to the physical limitations of field measurements, it is difficult to obtain leaf angles quickly and accurately, especially with a complicated canopy structure. An application of terrestrial LiDAR (Light Detection and Ranging) is a common solution for the purposes of leaf angle estimation, and it allows for the measurement and reconstruction of 3D canopy models with an arbitrary volume of leaves. However, in most cases, the leaf angle is estimated incorrectly due to inaccurate leaf segmentation. Therefore, the objective of this study was an emphasis on the development of efficient segmentation algorithms for accurate leaf angle estimation. Our study demonstrates a leaf segmentation approach based on a k-means algorithm coupled with an octree structure and the subsequent application of plane-fitting to estimate the leaf angle. Furthermore, the accuracy of the segmentation and leaf angle estimation was verified. The results showed average segmentation accuracies of 95% and 90% and absolute angular errors of 3° and 6° in the leaves sampled from mochi and Japanese camellia trees, respectively. It is our conclusion that our method of leaf angle estimation has high potential and is expected to make a significant contribution to future plant and forest research.