An experiment comprising of four levels of nitrogen viz., 0, 60, 90 and 120 kg ha-1 and three levels of sulphur viz., 0, 20 and 40 kg ha-1 was conducted infactorial randomized block design, replicated thrice during rabi 2016-17 at the Instructional Farm, Department of Agronomy, College of Agriculture, Junagadh Agricultural University, Junagadh to study the influence of N and S on yield and uptake of nutrients by fennel. The experimental medium deep black soil was clay in texture and had pH 8.06 and EC 0.41 dSm-1. Application of nitrogen @ 120 kg ha-1 and 40 kg S ha-1 individually had significant effect on yield and uptake of N, P, K, S, Fe, Mn, Zn and Cu by seed and stover. Application of 90 kg N ha-1 with 40 kg S ha-1 produced significantly higher seed and stover yield than the other treatments.