Introduction: Litsea glutinosa (Lour.) c.B.rob., is an aromatic medicinal tree belongs to the family lauraceae and rarely distributed in the Western ghats. ethnomedicinally, the bark is used by the traditional practitioners as a demulcent, emollient and in the treatment of diarrhea and dysentery, and to facilitate wound healing process. the leaf paste is applied to relieve respiratory diseases; cough and also used as a demulcent and an emollient. Methods: the petroleum ether, chloroform and ethanol extracts of stem bark and leaf were screened for potential antibacterial activity using agar well diffusion method against 8 clinical strains isolated from infectious sources belonging to gram-positive Staphylococcus aureus, Bacillus stubtilis and gram-negative Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Salmonella paratyphi and Proteus sp. Minimum inhibitory concentration (Mic) of the three sequential extracts of the leaf and stem bark was carried out against clinical isolated strains using two fold agar dilution method. Results: Ethanol extract of stem bark exhibited significant antibacterial activity with 2.5mg/ml MIC against Bacillus subtilis (19.20 ± 1.52), Escherichia coli (16.40 ± 0.55) and Staphylococcus aureus (15.20 ± 0.84) indicating the potent drug for the treatment of diarrhea and dysentery. among the tree sequential extracts of the leaf the ethanol extract showed potent antibacterial activity against Klebsiella pneumoniae (16.40 ± 0.80) and may acting as a suitable drug for respiratory disorders. Conclusion: the obtained results justify the ethnomedicinal claims of Litsea glutinosa.

Background: The use of chemical fertilizers to solve the problem of nutrient deficiency in soil has been associated with a number of environmental problems.Objective: The aim of this study was to perform an isolation and screening of native phosphofungi from mine soil. To evaluate the effect of phosphofungi on seed germination and seedling vigour index of Ground nut and Green gram.Materials and Methods: The phosphofungi were screened using Pikovskaya’s agar medium with tricalcium phosphate (Ca3 (PO4)2) as the phosphorus source. The soluble phosphorus, Phosphate solubilizing activity, titrable acidity, pH and fungal biomass were determined. Mineral phosphate solubilizing (MPS) activities of fungal isolates were tested in tricalcium phosphate medium.Results: Five promising phosphate solubilizing fungal species were screened. Aspergillus niger (13±1.0 mm) and Aspergillus flavus (8±0.6mm) showed the more phosphate solubilising index than compared to other fungal isolates. Analyzing the possible phosphorus released, from 3rd, 6th, 9th, and 12th day of incubation. Antagonistic effect of phosphofungi Aspergillus flavus (65±11 mm) and Aspergillus niger (60±13 mm) showed more effective against Colletotrichum sp. than compared to other fungal isolates. Aspergillus flavus (745±20µg/ml) and Penicillium spinulosum (600±20 µg/ml) showed maximum phosphate solubilizing activity on 3rd and 12th day of incubation. Fusarium redolens showed the more effectiveness on seed germination and seedling vigour than compared to other fungal isolates. Aspergillus flavus showed positive result for the production of Indole 3 acetic acid and remain four fungal strains are negative result.Conclusion: Biofertilizers from native phosphofungi could be used alongside reduced levels of inorganic fertilizers to enhance soil available Phosphorous.

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