Cancer is a major public health concern in both developed and developing countries. Anticancer activity refers to the ability of natural and synthetic, biological and chemical agents to revert, stop, or halt carcinogenic progression. To treat the ailment, several synthetic pharmaceuticals are utilised, but they have negative effects, thus researchers are investigating into plant-derived chemotherapeutic treatments. Enhalus acoroides, a member of the Hydrocharitaceae family, is found along the southeast coast of India and the tropical western Pacific. Hydroalcoholic extract of Enhalus acoroides inhibits the proliferation of Liver cancer and Breast cancer. The cell viability was analysed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, which shows that Hydroalcoholic extract of Enhalus acoroides significantly reduced the growth with IC50 value for HepG2 as 112.20μg/ml    and MCF-7 as 101.60μg/ml. Hence the results of current study conclude that Hydroalcoholic extract of Enhalus acoroides is a potent anticancer drug that can be utilized for the treatment of Hepatocellular cancer and Breast cancer.