By use of immunofluorescent techniques we found oral microorganisms on the surface of the sulcular epithelium and in the epithelial and connective tissues of the gingiva of individuals with periodontal disease. Anaerobic oral microorganisms frequently were found in these areas.

By the use of the peroxidase-labeled antibody method, significant localization of IgG, IgA, albumin and transferrin was demonstrated in the deep lesion of 20 carious teeth, where the secretory component was absent. These serum proteins formed a distinct zone, surrounding the overlying, shallow lesion infected with bacteria.

No abstract

Electrophoretic studies have shown that serum albumin, a,-, a2-, f0and y-globulin, and fibrinogen are present in the dental pulp fluid of the dog (

By use of immunohistochemical staining methods, salivary and serum components, ie, γ-globulin and serum albumin, were found in sound and carious human dentin. Most notable reactions were observed in dentinal tubules beneath a translucent zone (radiopaque zone) in carious dentin. Oral microorganisms also were found in soft carious dentin.