There is insufficient evidence to support screening of various tick-borne diseases (TBD) related microbes alongside Borrelia in patients suffering from TBD. To evaluate the involvement of multiple microbial immune responses in patients experiencing TBD we utilized enzyme-linked immunosorbent assay. Four hundred and thirty-two human serum samples organized into seven categories followed Centers for Disease Control and Prevention two-tier Lyme disease (LD) diagnosis guidelines and Infectious Disease Society of America guidelines for post-treatment Lyme disease syndrome. All patient categories were tested for their immunoglobulin M (IgM) and G (IgG) responses against 20 microbes associated with TBD. Our findings recognize that microbial infections in patients suffering from TBDs do not follow the one microbe, one disease Germ Theory as 65% of the TBD patients produce immune responses to various microbes. We have established a causal association between TBD patients and TBD associated co-infections and essential opportunistic microbes following Bradford Hill’s criteria. This study indicated an 85% probability that a randomly selected TBD patient will respond to Borrelia and other related TBD microbes rather than to Borrelia alone. A paradigm shift is required in current healthcare policies to diagnose TBD so that patients can get tested and treated even for opportunistic infections.

the present study evaluates the role of graphene oxide's (Go's) peroxidase-like and inherent/ carbocatalytic properties in oxidising silver nitrate (AgNO 3 ) to create graphene nanocomposites with silver nanoparticles (GO/Ag nanocomposite). Activation of peroxidase-like catalytic function of Go required hydrogen peroxide (H 2 o 2 ) and ammonia (nH 3 ) in pH 4.0 disodium hydrogen phosphate (na 2 Hpo 4 ). Carbocatalytic abilities of GO were triggered in pH 4.0 deionised distilled water (ddH 2 O). Transmission electron microscope (TEM), scanning electron microscope (SEM), cyclic voltammetry (CV) and UV-Vis spectroscopy aided in qualitatively and quantitatively assessing GO/Ag nanocomposites. teM and SeM analysis demonstrated the successful use of Go's peroxidase-like and carbocatalytic properties to produce GO/Ag nanocomposite. UV-Vis analysis indicated a higher yield in optical density values for GO/Ag nanocomposites created using GO's carbocatalytic ability rather than its peroxidaselike counterpart. Additionally, CV demonstrated that GO/Ag nanocomposite fabricated here is a product of an irreversible electrochemical reaction. Our study outcomes show new opportunities for GO as a standalone catalyst in biosensing. We demonstrate a sustainable approach to obtain graphene nanocomposites exclusive of harmful chemicals or physical methods.Remarkable optical, thermal, mechanical, and electrical properties of graphene have captivated the imaginations of scientists worldwide 1-4 . Graphene is an ideal composite counterpart to create flexible electronics 5,6 , design batteries with enhanced storage capability 7 , prevent steel corrosion 8 , and shield aircraft from heat 9 . Real-world graphene applications have originated across several industries like the Inov-8 running trainers that employ thermal and mechanical qualities of graphene to improve their durability 10 . The famous sports equipment manufacturer, HEAD applied graphene to create stronger tennis rackets with lighter weight distribution 10 . The BAC-Mono formula racing car also uses graphene to boost strength and reduce the mass of its body parts by 20% 11 . Miniaturization of biochemical assays using graphene-based sensors for research and clinical purposes is now a reality with AGILER100 12

Mycotoxin exposure in humans is primarily assessed through its occurrence in external sources, such as food commodities. Herein, we have developed a direct competitive ELISA to facilitate the detection of aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin (FUM B1/B2), ochratoxin A (OTA), and zearalenone (ZEA) in human serum. The analytical validation of the assay followed practices endorsed by the international research community and the EU directive 96/23/EC in order to examine detection capability, recovery, and cross-reactivity. The assay demonstrated a lower limit of quantitation (LLOQ) for AFB1 [0.61 ng/mL (hereon ng/mL = ppb)], DON (19.53 ppb), FUM (4.88 ppb), OTA (19.53 ppb), and ZEA (0.15 ppb). Recovery from human serum for all mycotoxins spanned from 73% to 106%. Likewise, the specificity for monoclonal antibodies against cross-reactant mycotoxins ranged from 2% to 11%. This study compares the LLOQ and recovery values with commercial and emerging immuno-based methods for detecting mycotoxins in foodstuffs. The LLOQ values from the present study were among the lowest in commercial or emerging methods. Despite the differences in the extraction protocols and matrices, the recovery range in this study, commercial tests, and other procedures were similar for all mycotoxins. Overall, the assay detected AFB1, DON, FUM, OTA, and ZEA in human serum with excellent accuracy, precision, and specificity.

Microalbuminuria is an established cardiovascular risk indicator in diabetes, hypertension and the general population. There is lack of information on MAU in healthy obese Indian adults and an ongoing debate whether obese adults deserve targeted identification and clinical intervention for MAU and prediabetes. We aimed to screen the healthy obese, young (group I) and middle aged (group II) adults for prevalence of MAU and prediabetes and study its association with Framingham risk score. The study included 50 healthy obese young (20-30 years) and middle aged adults (31-50 years), attending the outpatient clinic of Dept. of Medicine for a duration of 2 months (July-August). The patients were screened for fasting blood sugar, lipid profile and MAU. Of the total patients 28 % had MAU, 32.14 % of which had prediabetes and 33.33 % had diabetes whereas 10 % were normoglycemic. The group I patients had 50 % cases of MAU and group II had 25 % patients with MAU. Group II 63.63 % pre-diabetics. The values of MAU obtained were correlated with age, gender, body mass index, systolic and diastolic blood pressure, FBS, waist to hip ratio using Pearson's Coefficient (p < 0.05). The 10 year CVD risk calculated using FRS in subjects with MAU was higher as compared to those without MAU. Thus we conclude that Indian, young and middle aged obese adults to be at a risk of prediabetes, MAU and CV risk warranting their routine screening for better clinical outcomes.

Context: Peak expiratory flow rate (PEFR) is an effort-dependent parameter, emerging from the large airways within about 100-120 msec of the start of forced expiration. It measures the degree of obstruction in the airways. A child from an asthmatic family, having significantly low PEFR values than its height and age matched peers, can be considered under impending asthma category. Aims: The present study was proposed to detect early stages of airway obstruction in school going children. Settings and Design: Observational study conceived in the department of Physiology, AIIMS, Jodhpur, Rajasthan. Methods and Material: Hundred students of age group 7-15 years participated in the study. A family history for presence/absence of asthmatic symptoms was taken from all subjects. After anthropometric examination, PEFR values were recorded in standing position, using the Mini Wright Peak Flow meter after demonstrating them the right procedure. Three measurements were taken and the highest reading was recorded. Statistical Analysis Used: Fisher’s exact test was done to calculate the two tailed ‘P’ value, Odd’s ratio and relative risk. Results: Thirty-one Percent children with family history of airway obstruction showed PEFR values below 80% of the predicted value, while 5.74% who were not having any family history of asthma, also showed values below 80% of the predicted value. With Fisher’s exact test, the two tailed ‘P’ value was 0.0155 (significant). Conclusion: The results of this study support the vital role of PEFR related to changes in airflow, which eventually can result in early identification of children with airway obstruction.