Three new alkaloids (1–3), one new diphenyl ether (4) and fifteen known alkaloids (5–19) were isolated from the rattan stems of Sinomenium acutum. Comprehensive analyses of HR‐ESI‐MS, 1D (1H and 13C), 2D‐NMR (1H‐1H COSY, HSQC, HMBC, NOESY), circular dichroism (CD), UV and IR revealed the structures and absolute configurations of these new compounds. The structures of other compounds were determined by comparison of their 1H and 13C‐NMR data with previous literature reports. By measuring the amount of NO produced, the anti‐inflammatory properties of the isolated compounds were studied. The results showed that compounds 4 and 5 had strong NO inhibitory activity.

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