As an ecotone, the region between the Amazon Rainforest and Tropical Savanna (Cerrado) biomes is, by definition, more susceptible to climate change. Therefore, understanding palaeoenvironmental dynamics is essential to address the future responses of such transition areas to climatic fluctuations. In this context, we present a new sediment record for the Late-Holocene retrieved from Barro-Preto, currently an oxbow lake located in an ecotone at the southern Brazilian Amazon border. Our multi-proxy data include carbon and nitrogen isotopes, as well as bulk TOC, chlorophyll derivatives, grain-size and microcharcoal analyses, all anchored on a radiocarbon-dated chronology. The sedimentary process recorded at the Barro-Preto Lake responded to both local and regional climate dynamics. It was influenced by river excursions associated to local responses to precipitation changes by the activation of the palaeochannel connecting the main-stem river and the Barro-Preto lake. This activation was evidenced by the presence of different colour lithology laminations accompanied by coarser sediments and also by climate conditions known to influence the Amazon region. Depositional processes linked to lake dynamics and different oxbow lake cycle stages were also important to explain the changes verified in the Barro-Preto record, endorsing the use of this lake formation for palaeoclimatic reconstructions. The record indicated a rising humidity trend, reflected by a progressive increase in lacustrine productivity, in accordance to other studies carried out in the Amazon region concerning the Late-Holocene, associated with a more southward displacement of the Intertropical Convergence Zone. Despite this rising humidity trend, dry episodic events during the Late-Holocene were evidenced by charcoal data, also coherent with regional Amazon studies, albeit exhibiting increased intensity, suggesting that the transitional nature of the environment might have influenced susceptibility to fires.