Antihemoroid® suppository has been produced commercially by PT. Kimia Farma, Indonesia. For QC purposes, a separated densitometric method for analysis of its active ingredients, lidocaine hydrochloride and hexachlorophene, was applied. The objective of this study was obtaining more efficient analysis method of LH and HC, therefore an HPLC procedure has been developed for the determination of both compounds simultaneously. AYMC-Triart C18 column was used with a gradient mobile phase consisting of acetonitrile and phosphate buffer 0.05 M (pH 6.0). Quantitative evaluation was performed at 220 nm. Method validation was performed according to the new methods of USP 41. Result showed that the HPLC method was simple, accurate, precise, and robust. The HPLC method can be applied in simultaneous determination of LH and HC in suppositories as a QC tool in the pharmaceutical industries.

In this study, the HPLC and Total Organic Carbon (TOC) analysis methods have been developed and validated for the determination of the amount of Mometasone furoate and CIPioo residues on the surface of production equipment and to confirm the efficiency of the cleaning procedure. Mometasone furoate and CIPioo have been chosen based on the worst case assessment approach. The limit of mometasone furoate contamination that has been determined is 100 ug/swab and the specified limit of CIPioo contamination is 100 ug/swab which should not be exceeded during sequential cleaning of the equipment. Mometasone furoate shows good linearity in the range 0.1-1.0 ppm for the HPLC method and CIPioo 2.0-10.0 ppm for TOC Analysis. The percentage of recovery from stainless steel plates using swab sampling techniques was found in the limits of 95.12% and 99.93% respectively in the HPLC and TOC methods. Both methods are simple, inexpensive, short analysis time and high sensitivity for quantitative determination of Mometasone furoate and CIPioo on the surface of manufacturing equipment well below the contamination limit. The validated method meets the requirements for demonstration of the validation of residual cleaning of mometasone furoate and CIPioo on the surface of production equipment

Telah dikembangkan kromatografi cair kinerja tinggi (KCKT) fase balik yang sederhana, akurat, dan presisi untuk menetapkan campuran chlorpheniramin maleat, ephedrin HCl dan bromhexin HCl dalam sediaan sirop. Pemisahan diperoleh dengan menggunakan kolom Eurospher-100 C18 (ODS) 5μm (125 x 3 mm i.d.), dengan fase gerak etanol-asam asetat 0,5% yang dialirkan secara gradien dan detektor ultra violet. Kemampuan sistem memisahkan puncak-puncak analit cukup baik. Metode yang dipakai menghasilkan linieritas pada rentang 84,16–336,64 ppm untuk chlorpheniramin maleat, 160,56-642,24 ppm untuk ephedrin HCl dan 160,68–642,72 ppm untuk bromhexin HCl dengan. Batas kuantitasi ephedrin HCl, bromhexin HCl, dan chlorpheniramin maleat dalam sediaan sirop berturut-turut adalah 98,48; 109,73 dan 330,42 ppm. Akurasi terbaik untuk ephedrin HCl, bromhexin HCl, dan chlorpheniramin maleat dalam matrik sirop berturut-turut 96,83; 98,34 dan 97,24 % dengan presisi terbaik berturut-turut 0,39; 0,35 dan 2,25 %.

<p><strong><em>ABSTRACT</em></strong></p><p><strong><em> </em></strong><em>This study was conducted with the aim of knowing the level of calcium in guppy fish taken from the ditch in Kalirungkut Village area and also know the existence of guppy fish potency to be used as source of raw material of animal feed in the form of fish meal. Method of destruction of sample used is wet destruction by using HNO3 and HCl. For qualitative analysis and analysis of sample rate used ICPS instrument. From qualitative analysis yields peak at wavelength 393,366 nm which proves existence of calcium in fish sample. From the analysis of calcium levels gives results that guppy fish contain calcium with levels</em><em> </em><em>averaging 4.28%. From the analysis of calcium content in the sample then guppy fish potentially used as a source of raw material for animal feed seen from the calcium levels that have calcium levels of 2.5-5%</em><em>.</em></p><p> </p><p> </p><p><strong><em>ABSTRAK</em></strong></p><p><strong><em> </em></strong><em>Penelitian ini dilakukan dengan tujuan mengetahui kadar kalsium dalam ikan</em><em> </em><em>guppy yang diambil dari selokan di daerah Kelurahan Kalirungkut serta</em><em> </em><em>mengetahui adanya potensi ikan guppy untuk digunakan sebagai sumber bahan</em><em> </em><em>baku pakan ternak dalam bentuk tepung ikan. Metode destruksi sampel yang</em><em> </em><em>digunakan adalah destruksi basah dengan menggunakan HNO3 dan HCl. Untuk</em><em> </em><em>analisis kualitatif dan analisis kadar sampel digunakan instrumen ICPS. Dari</em><em> </em><em>analisis kualitatif menghasilkan puncak pada panjang gelombang 393,366 nm</em><em> </em><em>yang membuktikan adanya kalsium dalam sampel ikan. Dari analisis kadar</em><em> </em><em>kalsium memberikan hasil bahwa ikan guppy mengandung kalsium dengan kadar</em><em> </em><em>rata-rata 4,28%. Dari hasil analisis kadar kalsium dalam sampel maka ikan guppy</em><em> </em><em>berpotensi digunakan sebagai sumber bahan baku pakan ternak dilihat dari kadar</em><em> </em><em>kalsium yang mempunyai persyaratan kadar kalsium 2,5-5%.</em><em> </em></p><p> </p>