The influence of donor plant growth conditions on microspore embryogenesis in rapeseed (Brassica napus) was metric measurements of the microspores isolated from 2.5-to 5.0-millimeter buds showed that the microspores isolated from low-temperature-grown plants had significantly lower log 90-degree light scatter to forward angle light scatter and log 90-degree light scatter to time of flight ratios than those isolated from high-temperature-grown plants, suggesting that the former are more translucent than the latter. Thus, the effect of donor plant growth temperature on microspore embryogenesis may be mediated by a change in the physiology of the microspore cell, which results in the reduction of its cytoplasmic granularity and/ or exine density.Haploid plants can be produced by culturing anthers or isolated microspores of rapeseed (Brassica napus) (3, 9, 12, 13). Microspore to haploid embryo conversion frequencies as high as 10 to 50% have been reported (9, 16), but for most genotypes <1% of the microspores that are cultured will develop into embryos.Previous research with several plant species has shown that the conditions under which the donor plants were grown affect the frequency of androgenesis in culture. Seasonal variations in anther response were reported for a number of species including Solanum tuberosum (4), Hordeum vulgare (2), and Triticum aestivum (17). For B. napus, controlled studies of the effects of growth temperature, light intensity, and light duration on anther culture response showed that embryo production could be significantly increased by growing the donor plants at a low (1 5C) temperature (3,11,12) and high light (12,20). There is no detailed report of the 'Supported by the Ontario Ministry of Agriculture and Food.