There is concern that the use of sodium hypochlorite (NaOCl) and RC-Prep may lower the bond strength of resin cements. The objective of this study was to evaluate the effect of 5% NaOCl and RC-Prep treatment on the bond strength of a resin cement, C&B Metabond. Control roots (group 1) were biomechanically prepared using 0.9% NaCl as an irrigant; group 2, roots with 5% NaOCl; group 3, roots with RC-Prep; group 4, roots with 0.9% NaCl followed by 10% ascorbic acid; group 5, roots with 5% NaOCl followed by 10% ascorbic acid (pH 4); group 6, roots with 5% NaOCl followed by 10% neutral sodium ascorbate; and group 7, roots with RC-Prep followed by 10% ascorbic acid. All roots were then filled with C&B Metabond, incubated in water for 24 h, and then cross-sectioned into six 1-mm thick slabs representing cervical and middle root dentin. The slabs were trimmed and tested for tensile bond strength. The results demonstrated that both 5% NaOCl and RC-Prep produced significantly (p < 0.05) large reductions in resin-dentin bond strengths, and the reductions could be completely reversed by the application of either 10% ascorbic acid or 10% sodium ascorbate.

Adhesion of endodontic sealers to dentin and gutta-percha offers clues into their interaction with the wall of the root canal and the filling material. In this in vitro study, four classes of endodontic sealers (Kerr, a ZOE-based sealer; Sealapex, a calcium hydroxide-based sealer; AH 26, an epoxy resin-based system; and Ketac-Endo, a glass-ionomer based sealer) were compared for their ability to bond to dentin or gutta-percha. Flat coronal dentin or gutta-percha surfaces were created by using a diamond-impregnated saw blade. Aluminum cylinders (ca. 5-mm diameter) were stabilized on the substrates with small amounts of wax and then filled with one of the sealers. After setting in 100% humidity for 24 h, their tensile bond strengths were measured. Controls were the unfilled cylinders stabilized with wax. The bond strengths and modes of failure were measured. The results were statistically analyzed by using a two-way ANOVA (materials versus substrates) and Student-Newman-Keuls test at alpha = 0.05. The results indicated that sealant bond strengths to dentin (from lowest to highest mean +/- SD, n = 10) were: Kerr 0.13 +/- 0.02; Sealapex 0.30 +/- 0.08; Ketac-Endo 0.80 +/- 0.24; AH 26 2.06 +/- 0.53 MPa. The latter two were significantly different (p < 0.05) from the first two sealers and from themselves. The sealant bond strength to gutta-percha (from lowest to highest mean +/- SD, n = 10) were: Ketac-Endo 0.19 +/- 0.01; Sealapex 0.22 +/- 0.01; Kerr 1.07 +/- 0.19; AH 26 2.93 +/- 0.29 MPa. AH 26 gave the significantly highest bonds to gutta-percha.

CPT1a (carnitine palmitoyltransferase 1a) in the liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which, under native conditions, could form a dimer of the trimers, creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed a combined approach of sizing by mass and isolation using an immunological method. Blue native electrophoresis followed by detection with immunoblotting and mass spectrometry identified large molecular mass complexes that contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC). Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a-specific antibodies immunocaptured not only CPT1a but also ACSL and VDAC, further strengthening findings with blue native electrophoresis and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.Long chain fatty acids, an important energy source in mitochondria, are oxidized in the matrix via ␤-oxidation. Long chain fatty acids are activated on the cytosolic side of the mitochondrial outer membrane (MOM) 2 by long chain acyl-CoA synthetase (ACSL) (1). To be metabolized, activated fatty acids, as well as other substrates, ions, and nucleotides, cross the MOM through the voltage-dependent anion channel (VDAC), also called mitochondrial porin.Historically, the MOM was considered to be a sieve through which small compounds passed unimpeded. That view is no longer tenable. In Trypanosoma brucei, which expresses only one VDAC, knock-out of VDAC leads to mitochondria that no longer oxidize substrates (2). However, with disruption of the outer membrane, the mitoplasts are fully capable of oxidizing substrates. These studies highlight the essential role of VDAC in mitochondrial substrate oxidation in transportation of small anion substrates through the MOM.We studied PA22, a 22-kDa polyanion VDAC inhibitor (3), in mitochondrial substrate oxidation and observed that ADPstimulated glutamate, succinate, (ϩrotenone), and palmitoylcarnitine (ϩmalate) oxidation rates were unaffected. However, oxidation of palmitate (ϩATP, Mg 2ϩ , CoA, carnitine, and malate) and palmitoyl-CoA (ϩcarnitine and malate) was inhibited at 1-2 nmol of PA22/mg of mitochondrial protein. These data indicate that PA22 is selective for the first two steps in fatty acid oxidation without affecting other substrates. Additionally, we showed that PA22 interacts with VDAC by demonstrating that binding of hexokinase to rat liver mitochondria was inhibited by PA22 (3). VDAC was initially identified as the hexokinase-binding protein (4).PA10, a 10-kDa polyanion VDAC inhibitor (5), decreases ADP-stimulated oxidation of glutamate, malate, and succinat...

Within the limit of this study, it is concluded that SBA technique achieved predictable clinical outcomes. The addition of absorbable membranes enhanced bone gain in thickness compared with membrane-treated sites.

BackgroundThe aim of this study was to investigate the physical properties and biological effects of an experimentally developed injectable premixed calcium-silicate root canal sealer (Endoseal) in comparison with mineral trioxide aggregate (MTA) and a resin-based sealer (AHplus).MethodsThe pH, solubility, dimensional change, flow, and radiopacity of the materials were evaluated. Biocompatibility was evaluated on the basis of cell morphology and a viability test using MC3T3-E1 cells. For evaluate inflammatory reaction, the tested sealers were implanted into dorsal subcutaneous connective tissue of Sprague Dawley rats. After 7 days, the implants with the surrounding tissue were retrieved, and histological evaluation was performed.ResultsEndoseal showed high alkalinity similar to that of MTA. The solubility of the tested materials was similar. The dimensional change and flow of Endoseal was significantly higher than that of other materials (P < 0.05). The radiopacity of Endoseal was lower than that of AHplus (P < 0.05). The biocompatibility was similar to those of MTA. Inflammatory reaction of Endoseal was similar with that of MTA, but lower than that of AHplus (P < 0.05).ConclusionsThe present study indicates that Endoseal has favorable physical properties and biocompatibility. Therefore, we suggest that Endoseal has the potential to be used as a predictable root canal sealer.