Purpose: The purpose of this study was to investigate the work conditions, reported stress, role conflict and job satisfaction of infection control nurses working in general hospitals. Methods: The subjects were 125 infection control nurses from 102 general hospitals. Data were collected using a questionnaire consisting of questions about general characteristics, job characteristics, work condition, stress, role conflict, and job satisfaction. Data were collected from July to September 2011 and analyzed by descriptive statistics, independent t-test, one-way ANOVA, Scheffé test, Pearson's correlation, and Stepwise multiple regression in SPSS/WIN 18.0. Results: Over fifty percent (56.0%) of infection control nurses reported the majority of their time was monitoring intensive units. The average reported stress was 7.20, role conflict was 3.67, and job satisfaction was 3.14. The factors which influenced job satisfaction were stress, enough information, annual income, and the number of hospital beds. Conclusion:The factors contributing to job stress of infection control nurses need to be specifically identified. Education and training for infection control personnel should be provided to enhance job satisfaction.