Oranges (Citrus sinensis) can provoke an allergic reaction in some individuals. The concentration of profilin (Cit s 2)the major allergen in orangesis unknown, but this knowledge could be useful for preventing the development of allergies. The aim of this study was to quantify the amount of Cit s 2 in Navel oranges and in different varieties of citrus fruit. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed using both monoclonal and polyclonal antibodies. Extracts of Navel oranges and 12 citrus fruits were tested using this ELISA. Cit s 2 levels in the Navel orange pulp and peel were 1.81 ± 0.74 and 1.19 ± 0.87 µg/g, respectively, with accumulation detected primarily in the pulp. These findings may help in the production of low-risk processed oranges for orange allergy sufferers through selection of orange varieties and parts with low profilin content during food processing.

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Gly m 4 is one of important soybean allergens, which has property to cross-react with birch pollen allergen. However, a universal method for quantifying soybean allergen is unavailable. We aimed to develop an LC-MS/MS-based method for Gly m 4 quantification. A recombinant Gly m 4 (rGly m 4) was used to plot a standard curve and conduct a recovery test. The recovery of rGly m 4 from soybean grain extracts was 128% and the intra-and inter-day coefficients of variation were 12.8% and 13.2%, respectively. The method had a limit of quantification of 0.1 µg/mL. Using a validated method, Gly m 4 content was found to range from 309 to 421 µg/g in soybean grains and 2.7-67 µg/g in soybean processed foods. The newly developed method demonstrated sufficient reproducibility, sensitivity and accuracy, and might be helpful for individuals with soybean allergy to control Gly m 4 intake from food products.

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