Transgenic lettuce plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with Bcl-2 gene, related to apoptosis. After culture and selection on MS medium a number of kanamycin-resistant plantlets were regenerated. Polymerase chain reaction, Southern blot analysis and Northern blot analysis were used to identify and characterize the transgenic plants with the integrated Bcl-2 gene. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. T1 progeny of 100 transgenic lettuce inbred lines were inoculated with Sclerotinia sclerotiorum. Expression of the Bcl-2 peptide in transgenic lettuce plants provides high levels of field resistance against Sclerotinia sclerotiorum, causal agent of the agronomically important fungal disease of lettuce.
Poster Presentations
P213
POSTERSThere was statistical significance between the presences of deficits in lower cranial nerves with the degree of tumor extension in the internal carotid artery.Conclusion: Functional impairment of the lower cranial nerves was observed in the pre-and the postoperative time of patients undergoing surgery for resection of the glomus jugulare tumors and that its frequency was directly proportional to the extent of the tumor and its relationship with internal carotid artery.Objective: Determine the incidence of increased intracranial pressure, measured by lumbar puncture opening pressure, in patients with spontaneous cerebrospinal fluid (CSF) otorrhea.
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