To achieve better reproductive management rapid and reliable diagnostic tools are compulsory. Quick pregnancy recognition not only reduces the calving interval but also provide timely cure of disorders and mishappenings. Cattle, the economic animal in India is well known for pregnancy losses and postpartum related difficulties. In absence of medical aids and awareness this situation is getting more prominent. Although variety of traditional methods are in practice since decades but none of them qualifies for ideal candidate for diagnosis. These methods fail on technical ground in specificity, sensitivity and accuracy. With advancement in Biotechnological applications in animal sector, a new hope for getting novel biomarkers for pregnancy diagnosis has been raised. This review attempts to highlight and compare conventional and innovative approaches to detect bovine pregnancy early with low mortality rate.