The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose -SMG or Lactose -Egg Yolk -LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 10 6 motile spermatozoa in a volume of 22 mL, cooled to 5°C and stored up to 48 hours in a container proposed by Palhares (1997). Sperm motility and vigor were assessed in fresh semen, after first semen dilution, before insemination, at 24 and 48 hours after storage. For the fertility evaluation, 44 mares were inseminated with semen stored for a period between 12 and 24 hours. The mares were inseminated on fixed days (Mondays, Wednesdays, and Fridays) after the detection of a follicle greater than a 30mm diameter in one of the ovaries through ovulation. Pregnancy diagnosis was performed on day 12 post-ovulation, using transrectal ultrasonography. Semen diluted in SMG showed superior sperm motility than LEY, at the Pre-AI evaluation (P<0.05). At 48 hours of storage, all donkeys had motility values between 45 and 53% for semen diluted in SMG, while only one donkey showed motility greater than 30% in the LEY treatment. The pregnancy rate/cycle for mares inseminated with semen diluted in SMG was superior than that obtained using LEY (56.52% vs 4.76%, respectively).Keywords: donkey, fractionated collection, cooled semen, fertility RESUMO Objetivou-se com o presente experimento avaliar o efeito de dois diferentes diluidores (leite em pó desnatado glicose -SMG ou lactose gema de ovo -LEY) sobre as características físicas e a fertilidade do sêmen asinino coletado de forma fracionada e resfriado a 5ºC. Para isso, quatro jumentos da raça Pêga foram utilizados como doadores de sêmen. A fração espermática rica do ejaculado foi diluída preparando-se doses inseminantes contendo 400 x 106 espermatozoides móveis em um volume de 22 mL, resfriadas a 5ºC e armazenadas por até 48 horas em contêiner proposto por Palhares (1997)

The first three jets of the sperm-rich fraction of Pêga jackasses were collected and assessed separately. Five fertile Pêga jackasses were used as semen donors and underwent fractionated semen collection, using an open model artificial vagina. The first three jets of the semen were collected separately and assessed for volume, sperm motility, vigor, concentration/mL of semen, and sperm morphology. These characteristics were compared between first, second and third jets and between jackasses. It was observed that the jet volume differed (P<0.05) between jackasses, although it was similar (P>0.05) between first, second and third jets. Sperm motility did not differ (P>0.05) between jets and jackasses. Vigor was similar (P>0.05) between jets of the same jackass, and only the first jet differed (P<0.05) between jackasses. The first, second and third jets of the sperm-rich fraction had decreased sperm concentrations (P<0.05) of 955.56, 725.56 and 280.56x 10 6 sperm/mL of semen, respectively. Sperm morphology differed between the first three jets only for the incidence of mid-piece defect, higher in the third one (4.26%), compared to the first (3.36%) and second (3.38%) ones. When comparing the morphological characteristics of the sperm-rich fraction between five jackasses, regardless of the jet, there were differences in the percentage of normal sperm, proximal cytoplasmic droplet, mid-piece and head defects.

Um experimento foi realizado visando avaliar o fornecimento de rações úmidas e de água de consumo e rações com edulcorante para leitões desmamados e seus efeitos sobre o desempenho até o 90kg de peso vivo. Foram utilizados 32 leitões Large White x Landrace, desmamados aos 21 dias, submetidos, durante a fase inicial I (21 a 42 dias de idade), a 8 tratamentos correspondentes à combinação dos fatores: forma de apresentação da ração (seca e úmida), tipo de ração (sem e com edulcorante) e água de consumo (sem e com edulcorante). Foram avaliados a ocorrência de diarréia até o 10º dia pós-desmame, o ganho diário de peso (GDP), o consumo diário de ração (CDR) e a conversão alimentar (CA) até os 90kg de peso vivo. O consumo diário de água (CDA) foi avaliado na fase inicial I. O delineamento foi em blocos ao acaso, com arranjo fatorial 2³, com quatro repetições, sendo as médias comparadas pelo teste de Tukey e a incidência de diarréia pelo teste de qui-quadrado. Foram observadas diferenças (P<0,05), na fase inicial I, para o CDA para os grupos tratados com ração seca e para o CDR para os animais que receberam água com edulcorante. A ocorrência de diarréia foi maior (P<0,05) para os animais tratados com ração seca. O desempenho até os 90 kg de peso vivo foi semelhante entre os fatores (P>0,05). O experimento demonstrou que os tratamentos dirigidos na fase pós-desmame são insuficientes para melhorar os resultados até os 90kg de peso vivo.

Semen from the first 15mL of the ejaculate (P1) obtained from two boars (30mL) was diluted in glycineegg yolk extender, cooled at 5°C in a special container and rediluted in standard doses of 3x10 9 mobile spermatozoa after 12h of storage. Semen was also stored up to 24h after redilution. The physical characteristics of the semen were evaluated at different storage periods (fresh, 0h, 12h, rediluted, 24h, and 36h). The reproductive performance of the boars and their fertility regarding the insemination of primiparous sows were also determined. Two treatments were used: T1-15B sows inseminated with semen originated from hyperconcentrated heterospermic doses (15x10 9 mobile spermatozoa per dose), rediluted after 12h of storage at 5°C for standard doses of 3x10 9 mobile spermatozoa per dose and stored at 5°C up to 24h after redilution (n=10); T2-3B sows inseminated with standard heterospermic doses (3x10 9 mobile spermatozoa per dose), stored at 5°C up to 36h after semen collection (n=10). There was no effect (P>0.05) of treatments on the spermatic motility, even though a pronounced decrease (P>0.05) of their values at 12h of storage was recorded. However, they remained higher than 70% until 36h. There was effect of treatments on spermatic vigour at 0h (P<0.05), when T1-15B vigour was higher. There was also effect of the storage period for both treatments with a progressive decrease throughout 36h of storage, although the differences were not always significant. Pregnancy rates (90%) and the number of total farrowed piglets (15, 11-T1-15B; 13, 44-T2-3B) did not differ (P>0.05) between the treatments. It was concluded that the semen hyperconcentration of 15 billion of mobile spermatozoa per dose, stored at 5°C for 12h, did not result in drawbacks considering the physical characteristics of the semen, maintaining the pregnancy rates and prolificacy of the inseminated sows.Keywords: swine, fractioned collection, cooling, transport (15, 11 -T1-15B; 13, 44 -T2-3B) RESUMO

PALAVRAS CHAVE ADICIONAISAlimento alternativo. Análise econômica. Desempenho zootécnico. Suíno. ADDITIONAL KEYWORDSAlternative feedstuff. Economical analysis. Pigs. Zootechnical performance. RESUMOUtilizou-se 32 leitões machos castrados e fêmeas de linhagem comercial, desmamados com 21 dias de idade e peso vivo médio de 6,20±0,92 kg, objetivando avaliar o desempenho e a avaliação econômica de níveis inclusão de farelo de coco (FC) em rações para leitões na fase de creche. Os animais foram distribuídos em um delineamento em blocos ao acaso, com quatro tratamentos (0, 7, 14 e 21 % de farelo de coco), oito repetições por tratamento e um animal por unidade experimental. Observou-se que dos 21 aos 42 dias de idade, a inclusão de níveis acima de 7 % de farelo de coco na ração resultou em efeito linear negativo (p<0,05) sobre o ganho de peso diário. Dos 43 aos 63 dias de idade e no período total (21 a 63 dias de idade), observou-se feito quadrático dos níveis de FC nas rações (p<0,05) sobre a conversão alimentar, estimando-se melhor nível de 14,33 e 13,99 %, respectivamente. Para a fase dos 21 a 42 dias de idade, não houve efeito da inclusão de farelo de coco sobre as variáveis econômicas avaliadas. No entanto, dos 43 aos 63 dias e no período total (21 a 63 dias), observou-se efeito quadrático para as variáveis econômicas em função dos níveis de farelo de coco nas rações (p<0,05), com melhores resultados ao nível estimado aproximado de 15 %. Recomenda-se a inclusão de até 7 % de farelo de coco em rações para leitões dos 21 aos 42 dias de idade. Para a fase posterior, dos 43 a 63 dias de idade, o nível estimado de 15 % resultou em melhores índices de desempenho e econômico. Recomenda-se a inclusão de até 7 % de farelo de coco em rações para leitões dos 21 a 42 dias de idade. Para a fase posterior, dos 43 aos 63 dias de idade, o nível estimado de 15 % resultou em melhores índices de desempenho. SUMMARYA total of 32 male castrated boars and gilts of a commercial pig line aged 21 days and 6.20±0.92 kg of live weight were used to evaluate the performance and economical evaluation of copra meal inclusion in diets for piglets at nursery. Animals were allotted in a completely randomized block design with four treatments (0, 7, 14 and 21 % of copra meal), eight replicates per treatment and one animal as experimental unit. From 21 to 42 days of age, levels up to 7 % reduced daily weight gain of piglets (p<0.05). A quadratic trend on feed:gain ratio was observed from 42 to 63 days and total period (p<0.05), with better results at estimated level of 14.33 and 13.99 %, respectively. No effect of copra meal inclusion on economical variables was observed from 21 to 42 days of age. From 43 to 63 days and total period (21 to 63 days) a quadratic effect on economical indexes was observed with the inclusion of different levels of copra meal, with better results around 15 %. The inclusion of copra meal until the level of 7 % is recommended in piglet diets from 21 to 42 days of age. From 43 to 63 days, the estimated level of 15 % results i...