Growth rate, soluble protein content, osmotic stress and digestive enzyme activity were studied in early Litopenaeus schmitti postlarvae under different feeding regimens, by partially or completely replacing Artemia nauplii with Moina micrura. Growth was significantly higher in the postlarvae fed with a mixture of M. micrura, Artemia nauplii and algae (0.030 mg dry weight (dw) larva )1 day )1 , 17.4 ± 2.1% day )1 ), together with the postlarvae fed on Artemia nauplii and algae (0.027 mg dw larva )1 day )1 , 18.3 ± 1.9% day )1 ). Complete replacement of Artemia nauplii by M. micrura produced the lowest growth rate (0.018 mg dw larva )1 day )1 , 14.3 ± 1.6% day )1 ) and induced the highest protease and a-amylase activities and lower soluble protein contents. No significant difference among the treatments could be detected in postlarval resistance to osmotic stress. Based on the growth results, soluble protein content, enzymatic activity and osmotic stress resistance, we determined that the partial replacement of Artemia nauplii by M. micrura did not affect the growth, the soluble protein content and the nutritional state in the postlarvae of L. schmitti. To our knowledge, this is the first reported use of M. micrura as feed for early postlarvae of L. schmitti.
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This work focused on determining the fatty acid (FA) composition of commonly discarded fish on the Spanish Mediterranean coast in winter and summer to assess their potential use as raw sources of very long-chain polyunsaturated fatty acids (VLCPUFAs). Total n-3 VLCPUFA content significantly varies depending on capture season, and values were higher in winter for Pomadasys incisus (1.36 g/100 g), Chelidonichthys lucerna (1.67 g/100 g) and Trachinus draco (2.04 g/100 g), while Pagellus acarne had larger n-3 PUFA amounts in summer (3.89 g/100 g). Generally for most species, monounsaturated FA, eicosapentaenoic acid (20:5n-3) and total FA had higher values in summer, while both the n-3 VLCPUFA fraction and DHA content were higher in winter. Knowledge of these changes allows the processes for their proper use as valuable PUFA sources to be adjusted. The discarded fish herein studied could be generally considered to be raw n-3 VLCPUFAs sources.
The Casco Viejo of Panama is an area with great potential to develop a pedestrian zone, as it is an attractive area for travelers to visit. For this development to be carried out safely, it is recommended that the site is analyzed based on superblock criteria to ensure pedestrians can move through the streets in a free and accessible way regardless of their physical condition. Hence, it is necessary to evaluate the current state of the road infrastructure, as we did in this research. The methodology consisted of field visits where information was gathered on the distribution of space so that ideal routes for pedestrian tourists could be ensured. We surveyed the state of the sidewalks, their physical condition, minimum widths, heights, their accessibility, and the presence of obstacles that block movement from one point to another. We also assessed the characteristics of the roads, safe crossings and signs, the current management of parking in illegal areas, and the system of public transport routes. The results describe the current problems, which are, firstly, the issue of poor pedestrian and cyclist mobility due to the lack of space available on public roads as a result of poorly parked cars and the placement of bollards. Secondly, free movement is impossible for the disabled due to the absence of ramps and railings, which prevents the integration of this group in the area.
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