plants of the genus Eucalyptus, cultivated in many countries, have great importance for the world economy. In Brazil, this culture occupies a total of 5.7 million hectares, but native and exotic insect pests can reduce its productivity. Thaumastocoris peregrinus carpintero & Dellapé (Hemiptera: Thaumastocoridae), an exotic Australian pest, damages Eucalyptus plants. Biological control using the egg parasitoid Cleruchoides noackae Lin & Huber (Hymenoptera: Mymaridae), Heteroptera predators and entomopathogenic fungi, such as Beauveria bassiana and Metarhizium anisopliae, have potential for managing T. peregrinus. chemical insecticides, including bifenthrin and acetamiprid + bifenthrin, also control this insect. the compatibility of chemical and biological control methods favors integrated pest management. the objective of this study was to evaluate the selectivity of commercial products based on B. bassiana, M. anisopliae and the chemical bifenthrin on the parasitoid C. noackae and its parasitism on T. peregrinus eggs. the selectivity test followed the standards recommended by the international organization for Biological control (ioBc). Beauveria bassiana has selectivity to parasitism as well as viability, but was slightly harmful to C. noackae adults; M. anisopliae was innocuous to adults and to the viability of the offspring of this parasitoid, but it reduced the parasitism rate; and bifenthrin did not show selectivity to this parasitoid. The area of commercially planted forests in the world increased from 167.5 to 277.9 million hectares from 1990 to 2015 1. Brazil presently has 5.7 million hectares of Eucalyptus plantations with 24%, 17% and 15% of them in the states of Minas Gerais, São Paulo and Mato Grosso do Sul, respectively. The wood from these plantations is mainly destined for the pulp industry, with a production of 21 million tons in 2018 2,3. Insect pests of Australian origin detected in planted forests during the last three decades on a global scale may reduce Eucalyptus productivity 4. The bronze bug, Thaumastocoris peregrinus Carpintero & Dellapé (Hemiptera: Thaumastocoridae), was first detected in Brazil in 2008 in the states of São Paulo and Rio Grande do Sul, and has since dispersed to other Eucalyptus-producing states 5. This insect develops and produces fertile offspring on most Eucalyptus plantations in Brazil 6. Thaumastocoris peregrinus perforates and causes silvering, tanning, drying and defoliation from Eucalyptus plants 7. Biological control is the most widely-used method for managing T. peregrinus 8. This method includes the egg parasitoid Cleruchoides noackae Lin & Huber (Hymenoptera: Mymaridae), imported from Australia 8,9 , the predators Atopozelus opsimus Elkins (Hemiptera: Reduviidae) 10 and Supputius cincticeps Stäl (Heteroptera: Pentatomidae) 11,12 and entomopathogenic fungi 13,14. Beauveria bassiana and Metarhizium anisopliae, registered

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Cleruchoides noackae (Hymenoptera: Mymaridae), native to Australia, is the most promising biological control agent for Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae), an exotic Eucalyptus spp. pest in Brazil. The aim of this study was to determine the courtship behaviour, mating and oviposition of unmated or mated C. noackae females parasitizing T. peregrinus eggs utilizing the same rearing system used in biological control programmes in Brazil. The mating behaviour of eleven C. noackae unmated couples was observed and the time taken for males and females to find each other in polystyrene vials and the duration and number of copulations were recorded. Ten unmated or mated females were placed individually in vials with 10 T. peregrinus eggs each, and oviposition behaviour, percentage of eggs inserted and parasitized, viability and sex ratio of emerged C. noackae were recorded. This species lacked defined courtship behaviour and mated in less than an hour after adults' emergence. The time spent finding the first host, evaluating and inserting the ovipositor was similar for mated and unmated C. noackae females, as well as the frequency of inserted and parasitized eggs and their viability. Mated females took less time to find other host eggs and the sex ratio is female-biased. Occurrence of arrhenotokous parthenogenesis was confirmed. The ability of C. noackae to mate and lay eggs in less than one hour and parasitism of T. peregrinus eggs by females can improve the parasitoid mass rearing and biological control of T. peregrinus.

Insect pests introduced in eucalyptus plantations in Brazil are mostly of Australian origin, but native microorganisms have potential for their management. High quality biopesticide production based on entomopathogenic fungi depends on adequate technologies. The objective of this study was to evaluate Mycoharvester® equipment to harvest and separating particles to obtain pure Metarhizium anisopliae conidia to manage Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera: Thaumastocoridae). The Mycoharvester® version 5b harvested and separated M. anisopliae spores. The pure conidia were suspended in Tween 80® (0.1%) and calibrated to the concentrations of 1 x 106, 107, 108 and 109 conidia/ml to evaluate the pathogenicity, lethal concentration 50 and 90 (LC50, LC90) and lethal time 50 and 90 (LT50, LT90) of this fungus to T. peregrinus. This equipment harvested 85% of the conidia from rice, with production of 4.8 ± 0.38 x 109 conidia/g dry mass of substrate + fungus. The water content of 6.36% of the single spore powder (pure conidia) separated by the Mycoharvester® was lower than that of the agglomerated product. The product harvested at the concentrations of 108 and 109 conidia/ml caused high mortality to T. peregrinus third instar nymphs and adults. The separation of conidia produced by solid-state fermentation with the Mycoharvester® is an important step toward optimizing the fungal production system of pure conidia, and to formulate biopesticides for insect pest management.

Cleruchoides noackae Lin & Huber (Hymenoptera: Mymaridae) was imported to Brazil in 2012, to manage the exotic pest Thaumastocoris peregrinus Carpintero & Dellapé (Hemiptera: Thaumastocoridae), which has been damaging eucalyptus plantations. Knowledge of the thermal requirements and the fertility life table of C. noackae is important to improve mass rearing methods for this parasitoid and the effectiveness of its release to manage T. peregrinus. The objective was to evaluate the development period, thermal requirements and the fertility life table of C. noackae at different temperatures. The egg-adult period of this parasitoid varied from 43 to 14 days at 15 °C and 30 °C, respectively. The emergence of C. noackae adults was higher at 15 °C, 18 °C, 21 °C and 24 °C than at 30 °C. Female and male C. noackae need 226.75 and 230.41 degree-days and temperatures higher than 10.06 °C and 9.90 °C, respectively, to complete egg-adult development. The number of parasitized eggs per C. noackae female was higher at 21 °C, 24 °C and 27 °C, with 5.82, 7.73 and 5.50 eggs, respectively, than at 30 °C (0.45). Cleruchoides noackae longevity was greater at 15 °C, 21 °C and 24 °C. The net reproductive rate of the parasitoid was higher at 21 °C and 24 °C than at 30 °C, 3.05, 4.70 and 0.16, respectively. The finite rate of increase of C. noackae was greater at 21 °C, 24 °C and 27 °C, than at 30 °C and the intrinsic rate of increase was negative at 30 °C, −0.100. The temperatures 21 °C and 24 °C and from 18 °C to 27 °C are the most adequate for the reproduction and population increase of C. noackae parasitizing eggs of T. peregrinus, respectively.