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and ionic strength 0.02, w = 0.065 in the native state and w = 0.023 in the expanded state. These values of w require that the configurational change, at this temperature and ionic strength, at pH 3.5, should be accompanied by the binding of 29 protons per 67,000 g. If allowance is made for the fact that the radius of the expanded form is probably larger at ionic strength 0.02 than at ionic strength 0.04 (cf. serum albumin5), then the calculated proton uptake is increased to about 34. It thus appears that most, and perhaps all of the observed uptake of 36 protons can be accounted for.Ferrihemoglobin used in this study was prepared from crystalline horse oxyhemoglobin (kindly furnished by Dr. J. H. Wang) by the method of Steinhardt and Zaiser.8 The author is indebted to Dr. E. P. Geiduschek and Mr. G. Saliba for invaluable assistance in performing these measurements.

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Both cw-dichloroethylene and vinylidenc chloride reacted with disodium cysteinate in liquid ammonia to give the same dithioether I in good yield, but Zro#s-dichloroethylene failed to react. Trichloroethylene reacted to give only a monothioether II, while tetrachloroethylene gave both a mono-(III) and a dithioether (IV). The hyperconjugation and conjugative effect of a vinyl group attached to a sulfur atom is evidenced by a bathochromic shift in the ultraviolet which is accentuated by the presence of chlorine atoms on the vinyl group. The trichloroethylene derivative II is unique in giving a typical mustard gas color test and in exhibiting a high order of toxicity which is not necessarily a function of chlorine beta to sulfur.(3) (a) L.