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The effect of ascorbic acid (AA) on the formation of nitric oxide (NO) in peritoneal macrophages of SHK line mice was studied. ESR and UV spectroscopy revealed a significant increase in oxide formation in a suspension of macrophages with AA incubated at room temperature. In ESR studies, hemoglobin added to a suspension of macrophages was used as a NO trap, and the resulting nitric oxide was detected by the formation of hemoglobin complexes Heme-NO. The quantitative assessment showed that under these conditions, under the action of AA, 1.5x109 molecules of NO per cell are produced in macrophages. The increase in nitric oxide synthesis under the action of AA was also established by UV spectroscopy in the supernatant after incubation of MP with AA. Considering the known data on the cytotoxic effect of NO on viruses and bacteria, it is assumed that the effect of increasing nitric oxide synthesis in peritoneal, tissue MP and leukocytes under the action of AA is the main in the preventive and therapeutic effect of AA in a number of diseases, such as a sick of cold.