No abstract

A deficiency of riboflavin occurs only when the diet is very poor and lacks many nutrients. A lack of riboflavin causes sores in the mouth and inflammation of the tongue. Several methodologies have been developed for the determination of riboflavin, but those methods have few disadvantages like long analysis time, consume a lot of reagents and expensiveness. Hence, to overcome these problems, the differential pulse Polarographic technique was proposed in this study. The DPP method was used for the quantitative analysis of riboflavin. The experimental conditions were optimised to obtain the best characterised peak in terms of peak height with analytical validation of the method. This method is compared with UV-VIS spectroscopy and photo fluorimetry. It was found that the proposed method is highly sensitive, precise and fast.

Riboflavin (vitamin B2) plays an important role in living processes as a precursor of coenzymes. The core structure of riboflavin with isoalloxazine ring participates in enzyme‐catalyzed electron transfer processes of many important metabolites. Several methodologies have been developed for the determination of riboflavin, but those methods have disadvantages like long analysis time, consumption of a lot of reagents, and expensiveness. Hence, to overcome these problems, the DPP technique is proposed in this study. The DPP method is used for the quantitative analysis of riboflavin. The experimental conditions are optimized to obtain the best characterized peak in terms of peak height with analytical validation of the method. The proposed methods are applied for the analysis of riboflavin in pharmaceutical samples and vegetables. The riboflavin is found to adsorb and undergo irreversible reduction reaction at the working mercury electrode.