Necrotic lesions surrounded by yellow areas on leaves followed by stem necrosis were observed on chrysanthemum plants cultivated in Atibaia, Sào Paulo State, Brazil. The host range, in vitro properties and particle morphology of the causal virus were typical of a Tospovirus. Serological studies demonstrated that the virus differed from tomato spotted wilt. tomato chlorotic spot, groundnut ringspot and inpatiens necrotic spot Tospoviruses. The virus isolate is thus possibly a representativeof a new serogroup of a new virus.

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Badnavirus in Bougainvillea spectabilis showing virus-like symptoms was identified by the presence of bacilliform particles, measuring 125-130 ¥ 30-40 nm in leaf-dip preparations and by analysis of its putative open reading frame 3 sequence. The virus, tentatively named Bougainvillea bacilliform virus (BBV), had the highest identities (up to 60%) with Spiraea yellow leaf spot virus, Gooseberry vein banding associated virus, Taro bacilliform virus, and Citrus yellow mosaic virus. In phylogenetic analysis, BBV clustered with Badnavirus putative species. Attempts to transmit the virus to several hosts failed. This is the first report of a new Badnavirus detected in Bougainvillea.

RESUMOThe present paper reports results of the effect of Potato virus X (PVX) on the contents of total phenols and alkaloids in leaves of Datura stramonium. A significant decrease in the contents of phenols and alkaloids was observed in leaves inoculated with PVX (X-I). However, there was an increase in the percentage of phenols in leaves rubbed with phosphate buffer (C1-I) and in leaves from the nodes immediately above, possibly induced by mechanical injury. Gas chromatography/mass spectroscopy revealed amounts of scopolamine Duarte, L.M.L.; Salatino, M.L.F.; Salatino, A.; Negri, G.; Barradas, M.M. Effect of Potato virus X on total phenol and alkaloid contents in Datura stramonium leaves. Summa Phytopathologica, v.34, n.1, p. [65][66][67] 2008 ABSTRACT in samples submitted to all treatments, except X-I, in which the amount of this alkaloid was low. High amounts of an unidentified compound (molecular ion m/z 302 and a prominent peak at m/z 129) were noted in extracts from leaves X-I, C1-I and leaves from the nodes immediately above the leaves inoculated with PVX. It is suggested that the synthesis and accumulation of the unidentified compound is a result of stress from mechanical injury and virus inoculation.O presente trabalho relata resultados sobre a ação do Potato virus X (PVX) no conteúdo de fenóis totais e alcalóides em folhas de Datura stramonium. Uma diminuição significativa no conteúdo dessas substâncias foi observada nas folhas inoculadas com o PVX (X-I). Entretanto, houve um aumento na porcentagem de fenóis nas folhas friccionadas com tampão fosfato (C1-I) e nas acima das friccionadas, possivelmente induzido por injúria mecânica. v.34, n.1, p.65-67, 2008 Palavras chave: Potexvirus, substâncias fenólicas, alcalóides tropânicos, plantas medicinais quantidades de escopolamina nas amostras submetidas a todos os tratamentos, exceto em X-I, no qual a quantidade deste alcalóide foi baixa. Altas quantidades de uma substância não identificada (íon molecular m/z 302 e um proeminente pico a m/z 129) foram notadas em extratos a partir de folhas dos tratamentos X-I, C1-I e nas acima das inoculadas com o PVX. Sugere-se que a síntese e acúmulo da substância não identificada é um resultado do estresse causado pela injúria mecânica e pelo vírus.