RNA interference is a useful tool to evaluate pathogenic mechanisms of M. canis. For the first time, a role in pathogenicity could be attributed to a protease of a dermatophyte, namely Sub3 from M. canis, which is required for adherence to but not for invasion of the epidermis.
The diagnosis of canine SNA is more likely to be confirmed following culture of mucosal biopsies or fungal plaques than nasal secretions sampled blindly with swabs. Incubating cultures at 37 degrees C is more likely to provide a diagnostic outcome than when samples are cultured at room temperature. Fungal culture of nasal specimens has good specificity for the diagnosis of SNA in dogs.
-Two successive batches of 32 fattening pigs per batch were each divided into 2 homogenous groups of 16 pigs fed either a high crude protein (CP) level diet (HP-groups) or a low crude protein level diet balanced with synthetic amino acids (LP-groups). Pigs were raised on strawbased deep litters in separate rooms according to diets. Once a month, the emissions of ammonia (NH 3 ), nitrous oxide (N 2 O), methane (CH 4 ), carbon dioxide (CO 2 ) and water vapour (H 2 O) were measured continuously for 6 days consecutively. The mean nitrogen (N) intakes of pigs from HPgroups and LP-groups were 6.83 kg and 5.78 kg per pig respectively with mean initial and final pig body weights of 26.6 and 111.4 kg. There was no significant difference between the daily weight gains with regards to the diet CP content. At the end of the fattening periods, the N-contents of the litters were on average 1.84 kg per pig for the HP-groups and 1.56 kg per pig for the LP-groups. Gaseous emissions in the room with LP-groups were, compared with the emissions in the room with HP-groups, 26.1% lower for NH 3 (10.60 vs. 14.35 g per pig per day), 12.8% lower for CH 4 (13.12 vs. 15.04 g per pig per day) and 2 times higher for N 2 O (1.02 vs. 0.52 g per pig per day). The emissions of CO 2 and H 2 O were not significantly different according to the diet CP level.fattening pig / crude protein / ammonia / nitrous oxide / methane Résumé -Effets d'une réduction de la teneur en protéine brute de l'aliment sur les émissions de gaz associées à l'élevage de porcs charcutiers sur litières accumulées. Deux lots successifs de 32 porcs charcutiers par lot ont été chacun subdivisés en 2 groupes de 16 nourris soit avec un aliment à haute teneur en protéine brute (groupes HP) ou un aliment à faible teneur en protéine brute complémenté avec des acides aminés synthétiques (groupes FP). Les porcs ont été hébergés sur des litières accumulées de paille, dans des locaux distincts en fonction de l'aliment reçu.
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