Harker, K. N., O'Donovan, J. T., Turkington, T. K., Blackshaw, R. E., Lupwayi, N. Z., Smith, E. G., Klein-Gebbinck, H., Dosdall, L. M., Hall, L. M., Willenborg, C. J., Kutcher, H. R., Malhi, S. S., Vera, C. L., Gan, Y., Lafond, G. P., May, W. E., Grant, C. A. and McLaren, D. L. 2012. High-yield no-till canola production on the Canadian prairies. Can. J. Plant Sci. 92: 221–233. Relatively high prices and increasing demand for canola (Brassica napus L.) have prompted growers to produce more canola on more cropland. Here we determine if canola seed yield and oil concentration can be increased over current levels with high levels of crop inputs. From 2008 to 2010, direct-seeded experiments involving two seeding rates (75 vs. 150 seeds m−2), two nitrogen rates (100 vs. 150% of soil test recommendation), and the presence or absence of polymer-coated nitrogen or fungicides, were conducted at eight western Canada locations in canola-wheat-canola or continuous canola rotations. Herbicides, insecticides and fertilizers other than nitrogen were applied as required for optimal canola production. Increasing recommended nitrogen rates by 50% increased canola yields by up to 0.25 Mg ha−1. High (150 seeds m−2) versus lower (75 seeds m−2) seeding rates increased canola yields by 0.07 to 0.16 Mg ha−1. Fungicide treatment or polymer-coated nitrogen blended with uncoated urea increased canola yields by 0.10 Mg ha−1 in 2010, but not in 2008. The highest canola input combination treatment following wheat (3.50 Mg ha−1) yielded substantially more than the same high input treatment following canola (3.22 Mg ha−1). Average site yields were influenced by site conditions such as soil organic matter, days to maturity, and temperature, but these site and environmental predictors did not alter treatment rankings. Using higher than the soil test recommended rate of nitrogen or planting 150 versus 75 seeds m−2 increased canola yields consistently across western Canada. Canola oil concentration varied among canola cultivars, but was consistently low when N rates were high (150% of recommended). Higher than normal seeding rates led to high canola seed oil concentration in some cases, but the effect was inconsistent.

Feng, J., Hwang, R., Chang, K. F., Conner, R. L., Hwang, S. F., Strelkov, S. E., Gossen, B. D., McLaren, D. L. and Xue, A. G. 2011. Identification of microsatellite markers linked to quantitative trait loci controlling resistance to Fusarium root rot in field pea. Can. J. Plant Sci. 91: 199–204. Fusarium root rot, caused by Fusarium solani (Mart.) Sacc. f. sp. pisi (F. R. Jones) W. C. Snyder & H. N. Hans, is the most common root disease of field pea (Pisum sativum L.) in western Canada. In this study, a recombinant inbred line (RIL) population (n=71) of field pea, derived from crosses between a resistant cultivar Carman, and a susceptible cultivar Reward, was evaluated to identify quantitative trait loci (QTL) controlling resistance to Fusarium root rot. The parental genotypes and RILs were evaluated for resistance to root rot following inoculation with F. solani in field experiments during 2007 and 2008. The frequency distribution of disease severities among the RILs was continuous. Transgressive segregation for resistance was observed among the RILs, with five lines more resistant than Carman, but no lines were more susceptible than Reward. To identify DNA markers linked with the resistance, 213 microsatellite markers were screened with genomic DNA from the two parental cultivars. Only 14 markers were polymorphic between the two parents and were used to genotype each of the RILs. Quantitative trait loci analysis based on the mean disease severity data from 2007 and 2008 identified a QTL that explained 39.0% of the phenotypic variance in the RIL population. This QTL is flanked by markers AA416 and AB60 on linkage group VII. The microsatellite markers that are closely linked to this QTL may be useful for marker assisted selection to develop cultivars with superior Fusarium root rot resistance.

Balasubramanian, P. M., Conner, R. L., McLaren, D. L., Chatterton, S. and Hou, A. 2014. Partial resistance to white mould in dry bean. Can. J. Plant Sci. 94: 683–691. White mould, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a constraint on dry bean (Phaseolus vulgaris L.) production across Canada. Under high disease pressure, dry bean cultivars succumb to the disease resulting in a severe loss of seed yield and quality. Disease development is highly influenced by environmental conditions. In the absence of complete resistance to white mould, dry bean cultivars with both field resistance (avoidance) and physiological resistance would be preferred by growers in order to reduce disease risk and production costs. The objective of this study was to characterize select dry bean genotypes for field resistance to white mould in inoculated disease nurseries, and physiological resistance in a controlled environment. White mould ratings ranged from moderately susceptible to susceptible. Dry bean cultivars lacked both field resistance and physiological resistance. Germplasm lines I9365-25, G122, A 195 and I9635-31 had low disease severity ratings (5.1 to 5.6 at 26 d after inoculation using a 1 to 9 visual disease rating scale) in controlled environment indicating physiological resistance. L 192, MO162, 92BG-7 and OAC Rico also had acceptable levels of physiological resistance (severity ratings of 5.8 to 6.2 at 26 d after inoculation). G122, A 195, L 192 and MO 162 had low white mould disease incidences (16 to 25%) in the field over 4 yr indicating field resistance to white mould. These genotypes may be used as parents in the development of dry bean cultivars with enhanced resistance to white mould.

Chang, K. F., Conner, R. L., Hwang, S. F., Ahmed, H. U., McLaren, D. L., Gossen, B. D. and Turnbull, G. D. 2014. Effects of seed treatments and inoculum density of Fusarium avenaceum and Rhizoctonia solani on seedling blight and root rot of faba bean. Can. J. Plant Sci. 94: 693–700. Production of faba bean cultivars with tannin-free seed on the Canadian prairies has potential for use in human food and as a feedstock for livestock and aquaculture. However, root rot is a major constraint to production. The effects of fungicide seed treatments on root rot caused by Fusarium avenaceum and Rhizoctonia solani, and the effect of inoculum density on seedling emergence, nodulation, root rot severity and yield of faba bean were examined across 12 station years from 2009 to 2011 at sites in Manitoba and Alberta. Fusarium avenaceum reduced seedling emergence more than R.solani, but both pathogens had a similar impact on seed yield. The effects of inoculum density and seed treatment were generally significant for seedling emergence, root rot severity and seed yield for both pathogens. The interaction effects of inoculum density×seed treatment for F.avenaceum and R.solani were only significant for seed yield and seedling emergence. Seedling emergence and seed yield declined with increasing inoculum level for both F. avenaceum and R.solani. Fungicidal seed treatments with Apron Maxx (fludioxonil+metalaxyl) and Vitaflo 280 (carbathiin+thiram) consistently improved emergence and seed yield in trials inoculated with F.avenaceum or R.solani. This study demonstrated that seed treatment to manage root rot of faba bean is warranted.