This stable-antigen solid-phase system readily detects and identifies red cell antibodies that are important in transfusion.

Objective: Development of a new solid–phase system for screening and identifying irregular red cell antibodies. Materials and Methods: Red blood cell membranes were prepared by a semi–automated procedure in which the hemolysate solution was passed through a hollow–fiber system. The membranes were fixed to the solid phase (microtiter plates) by centrifugation and incubated with 8% fat–free milk. Antibodies added to the microtiter plate were detected by anti–human antibodies adsorbed onto yellow latex particles. Results: The system had good sensitivity (titer <1); 97% of anti–D samples were detected. The detection system was stable for 6 months at 4°C. Conclusion: This stable–antigen solid–phase system readily detects and identifies red cell antibodies that are important in transfusion.